Odom Duncan T
Cancer Research UK, Li Ka Shing Centre, University of Cambridge, Cambridge, UK,
Subcell Biochem. 2011;52:175-91. doi: 10.1007/978-90-481-9069-0_8.
Recent technological developments have revolutionized our understanding of transcriptional regulation by providing an unprecedented ability to interrogate in vivo transcription factor binding. The combination of high-throughput sequencing with chromatin precipitation of transcription factors and specifically labeled histones has allowed direct protein-DNA contacts to be visualized across genomes as large and complex as mammals at base-pair resolution. This chapter reviews the developments that led to these insights, with particular focus on examples of early protein-DNA localization experiments using genomic microarrays in mammals and yeast. Four state-of-the-art research directions are highlighted as examples of previously unimaginable frontiers now under active investigation.
最近的技术发展彻底改变了我们对转录调控的理解，因为它提供了前所未有的能力来研究体内转录因子的结合情况。高通量测序与转录因子染色质沉淀以及特异性标记组蛋白的结合，使得在碱基对分辨率下，能够在像哺乳动物基因组这样庞大而复杂的基因组中直接可视化蛋白质与DNA的接触。本章回顾了促成这些见解的发展历程，特别关注在哺乳动物和酵母中使用基因组微阵列进行早期蛋白质-DNA定位实验的例子。作为目前正在积极研究的以前难以想象的前沿领域的例子，突出了四个最先进的研究方向。
