Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
J Clin Biochem Nutr. 2011 May;48(3):237-44. doi: 10.3164/jcbn.10-119. Epub 2011 Apr 13.
Resveratrol, a phytoalexin present in the skin of grapes and red wine, has been demonstrated to possess a wide range of health promoting activities including anti-diabetic properties. In the present study, we investigated the effect of resveratrol in both type 2 diabetic mice and cell culture systems. In cultured L6 myotubes, we studied the effect of resveratrol on glucose uptake and translocation of glucose transporter 4 to plasma membrane from the aspects of insulin signaling and AMP-activated protein kinase signaling. In cultured RIN-5F cells, we examined whether resveratrol would protect the pancreas-derived β-cells from oxidative stress. Resveratrol significantly suppressed the elevation in the fasting blood glucose level and the serum triglyceride and lipid peroxide levels in db/db mice. Resveratrol stimulated glucose uptake and glucose transporter 4 translocation by activating both insulin signaling and AMP-activated protein kinase signaling. Moreover, resveratrol could protect pancreatic β-cells from advanced glycation end products-induced oxidative stress and apoptosis. From these results, resveratrol is suggested to show anti-diabetic effect by stimulating both insulin-dependent and -independent glucose uptake in muscles and by protecting pancreatic β-cells from advanced glycation end products-induced oxidative stress and apoptosis.
白藜芦醇是葡萄和红酒皮中的一种植物抗毒素，已被证明具有广泛的促进健康的作用，包括抗糖尿病特性。在本研究中，我们研究了白藜芦醇对 2 型糖尿病小鼠和细胞培养系统的影响。在培养的 L6 肌管中，我们从胰岛素信号和 AMP 激活的蛋白激酶信号方面研究了白藜芦醇对葡萄糖摄取和葡萄糖转运蛋白 4 从细胞质膜向细胞膜转位的影响。在培养的 RIN-5F 细胞中，我们检查了白藜芦醇是否会保护胰腺衍生的β细胞免受氧化应激。白藜芦醇显著抑制 db/db 小鼠空腹血糖水平、血清三酰甘油和脂质过氧化物水平的升高。白藜芦醇通过激活胰岛素信号和 AMP 激活的蛋白激酶信号来刺激葡萄糖摄取和葡萄糖转运蛋白 4 的转位。此外，白藜芦醇可以保护胰腺β细胞免受糖基化终产物诱导的氧化应激和细胞凋亡。从这些结果可以看出，白藜芦醇通过刺激肌肉中胰岛素依赖和非依赖的葡萄糖摄取，并保护胰腺β细胞免受糖基化终产物诱导的氧化应激和细胞凋亡，从而显示出抗糖尿病作用。
