State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China, Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
Mol Ecol Resour. 2008 Nov;8(6):1454-6. doi: 10.1111/j.1755-0998.2008.02215.x. Epub 2008 Sep 15.
Eight polymorphic di- and trinucleotide microsatellite loci were developed in the Chinese scorpion, Mesobuthus martensii. The expected heterozygosity at these loci ranges from 0.019 to 0.860, with the observed allele numbers varying from two to 25. Overall, there were no deviations from Hardy-Weinberg equilibrium and no observed linkage disequilibrium after Bonferroni correction. Cross-species amplification of these loci in Mesobuthus eupeus revealed that five loci can amplify successfully in this species. The polymorphic microsatellite DNA markers reported here should provide helpful means to address questions concerning phylogeographical patterns and evolutionary history of M. martensii and closely related species.
在中国蝎子，Mesobuthus martensii 中开发了八个多态性二核苷酸和三核苷酸微卫星基因座。这些基因座的预期杂合度范围从 0.019 到 0.860，观察到的等位基因数量从两个到 25 不等。总体而言，经过 Bonferroni 校正后，没有偏离 Hardy-Weinberg 平衡，也没有观察到连锁不平衡。这些基因座在 Mesobuthus eupeus 中的种间扩增表明，这五个基因座可以在该物种中成功扩增。本研究报道的多态性微卫星 DNA 标记物应为解决 M. martensii 和相关物种的系统地理学模式和进化历史问题提供有益手段。
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