Department of Entomology, The Ohio State University - OARDC, 1680 Madison Avenue, Wooster, OH 44691, USA.
Mol Ecol Resour. 2009 Jan;9(1):207-9. doi: 10.1111/j.1755-0998.2008.02369.x. Epub 2008 Oct 22.
Twenty microsatellite loci were identified from genomic DNA enrichment and expressed sequence tags of entomopathogenic nematode Heterorhabditis bacteriophora. Eight loci were found to be polymorphic in a Northeast Ohio H. bacteriophora population. Levels of polymorphism were evaluated in 31-56 individuals and the number of alleles ranged from two to three. The values of observed and expected heterozygosities ranged from 0 to 0.536 and from 0.223 to 0.616, respectively. All eight loci showed heterozygote deficiencies, but three conformed to Hardy-Weinberg equilibrium at the subpopulation level. This is the first set of microsatellite markers in entomopathogenic nematodes.
从昆虫病原线虫异小杆线虫基因组 DNA 富集和表达序列标签中鉴定出 20 个微卫星位点。在俄亥俄州东北部的异小杆线虫种群中发现 8 个多态性位点。在 31-56 个人中评估了多态性水平，等位基因数范围为 2-3。观察到的和预期的杂合度值分别为 0 到 0.536 和 0.223 到 0.616。所有 8 个位点均表现出杂合子缺失，但有 3 个在亚种群水平上符合哈迪-温伯格平衡。这是昆虫病原线虫中第一组微卫星标记。
