源自腺样体组织原代培养的上皮细胞的爱泼斯坦-巴尔病毒感染。

Epstein-Barr virus infection of epithelial cells derived from primary cultures of adenoidal tissue.

作者信息

Furukawa M, Sakashita H, Kato C, Umeda R

机构信息

Department of Otorhinolaryngology, School of Medicine, Kanazawa University, Japan.

出版信息

Eur Arch Otorhinolaryngol. 1990;247(2):109-13. doi: 10.1007/BF00183179.

DOI:10.1007/BF00183179

PMID:2156536

Abstract

Epithelial cells derived from primary cultures of adenoidal tissue were exposed to Epstein-Barr virus (EBV) from the throat washings of a patient with infectious mononucleosis (IM) and P3HR-1 and B95-8 cell strains. They were then examined for EBV-specific antigens by immunofluorescence. EBV from both the P3HR-1 cell strains and the throat washings of the IM patient infected the epithelial cells at the 6th and 9th days, respectively. Although the EBV-antigen-positive cells did not increase in number and disappeared at the 12th day after infection, EBV antigens of the virus's replication cycle could still be detected in the epithelial cells shed from cell culture layer at this time. However, the virus obtained from the cell-free 12th day's culture medium was incapable of transforming cord blood lymphocytes.

摘要

将来自腺样体组织原代培养的上皮细胞，暴露于来自一名传染性单核细胞增多症（IM）患者咽漱液中的爱泼斯坦-巴尔病毒（EBV）以及P3HR-1和B95-8细胞株。然后通过免疫荧光检查它们是否存在EBV特异性抗原。来自P3HR-1细胞株和IM患者咽漱液中的EBV，分别在第6天和第9天感染了上皮细胞。尽管EBV抗原阳性细胞数量没有增加，并在感染后第12天消失，但此时仍可在从细胞培养层脱落的上皮细胞中检测到病毒复制周期的EBV抗原。然而，从第12天无细胞培养基中获得的病毒无法转化脐血淋巴细胞。