通过免疫荧光法诊断人冠状病毒感染：方法及其在住院儿童呼吸道疾病中的应用
Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalized children.
作者信息
McIntosh K, McQuillin J, Reed S E, Gardner P S
出版信息
J Med Virol. 1978;2(4):341-6. doi: 10.1002/jmv.1890020407.
Abstract
Rabbit antisera were prepared against coronavirus strains 229E and OC43 and used successfully to detect viral antigen in epithelial cells shed from the nasopharynx of symptomatic volunteers who had received coronavirus inocula three to four days before. The same serologic reagents were applied to nasopharyngeal secretion cells obtained from 106 infants and children hospitalized with respiratory tract disease and apparently not infected with conventional respiratory viruses. No coronavirus infections were detected by this method. It appears that coronavirus OC43 or 229E infections were not common in children in Tyneside hospitals during the period of study. However, fluorescence is a useful method for detection of coronavirus infections in symptomatic human subjects.
摘要
制备了针对冠状病毒株229E和OC43的兔抗血清，并成功用于检测有症状志愿者鼻咽部脱落上皮细胞中的病毒抗原，这些志愿者在三到四天前接种了冠状病毒。将相同的血清学试剂应用于从106名因呼吸道疾病住院且显然未感染传统呼吸道病毒的婴幼儿获取的鼻咽分泌细胞。通过这种方法未检测到冠状病毒感染。在研究期间，泰恩赛德医院的儿童中似乎未常见冠状病毒OC43或229E感染。然而，荧光法是检测有症状人类受试者冠状病毒感染的一种有用方法。
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本文引用的文献
1
CULTIVATION OF A NOVEL TYPE OF COMMON-COLD VIRUS IN ORGAN CULTURES.新型普通感冒病毒在器官培养中的培养
Br Med J. 1965 Jun 5;1(5448):1467-70. doi: 10.1136/bmj.1.5448.1467.
2
THE USE OF VOLUNTEERS.志愿者的使用
Am Rev Respir Dis. 1963 Sep;88:SUPPL 128-34. doi: 10.1164/arrd.1963.88.3P2.128.
3
Effects of a "new" human respiratory virus in volunteers.一种“新型”人类呼吸道病毒对志愿者的影响。
Br Med J. 1967 Sep 23;3(5568):767-9. doi: 10.1136/bmj.3.5568.767.
4
Seroepidemiologic survey of coronavirus (strain OC 43) related infections in a children's population.儿童人群中冠状病毒（OC 43株）相关感染的血清流行病学调查。
Am J Epidemiol. 1971 Jul;94(1):43-9. doi: 10.1093/oxfordjournals.aje.a121293.
5
Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease.从呼吸道疾病患者中分离出的新型病毒在气管器官培养中的复苏。
Proc Natl Acad Sci U S A. 1967 Apr;57(4):933-40. doi: 10.1073/pnas.57.4.933.
6
Protein composition of coronavirus OC 43.冠状病毒OC 43的蛋白质组成。
Virology. 1972 May;48(2):516-27. doi: 10.1016/0042-6822(72)90062-1.
7
Medical reviews. Coronaviruses.医学综述。冠状病毒。
Yale J Biol Med. 1974 Dec;47(4):234-51.
8
Coronavirus infection in acute lower respiratory tract disease of infants.婴儿急性下呼吸道疾病中的冠状病毒感染
J Infect Dis. 1974 Nov;130(5):502-7. doi: 10.1093/infdis/130.5.502.
9
Antigenic relationships amongst coronaviruses.冠状病毒之间的抗原关系。
Arch Gesamte Virusforsch. 1970;31(3):352-64. doi: 10.1007/BF01253769.
10
Seroepidemiologic studies of coronavirus infection in adults and children.成人和儿童冠状病毒感染的血清流行病学研究。
Am J Epidemiol. 1970 Jun;91(6):585-92. doi: 10.1093/oxfordjournals.aje.a121171.
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