Department of Environmental Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):806-810. doi: 10.1099/ijs.0.022814-0. Epub 2011 May 13.
Two non-motile, Gram-staining-negative, yellow-pigmented bacterial strains designated HMD1001T and HMD1033T were isolated from the water of a mesotrophic artificial lake in Korea. A phylogenetic tree based on 16S rRNA gene sequences indicated that both strains could be assigned to the genus Flavobacterium; strain HMD1001T appeared most closely related to Flavobacterium fluvii H7T (96.8 % sequence similarity), F. succinicans DSM 4002T (96.6 %) and F. hydatis DSM 2063T (96.6 %) whereas strain HMD1033T appeared most closely related to Flavobacterium psychrolimnae LMG 2201T (96.2 %), F. segetis AT1048T (96.2 %) and F. weaverense AT1042T (96.2 %). The major fatty acids of strain HMD1001T were iso-C15:0 (21.5 %), summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c; 18.0 %) and iso-C15:1 G (7.6 %), whereas those of HMD1033T were summed feature 3 (23.8 %), iso-C15:0 3-OH (16.9 %), iso-C15:0 (15.3 %) and anteiso-C15:0 (12.1 %). The genomic DNA G+C contents of strains HMD1001T and HMD1033T were 35.9 and 32.2 mol%, respectively. Phylogenetic and phenotypic evidence indicates that strains HMD1001T and HMD1033T represent two novel species of the genus Flavobacterium, for which the names Flavobacterium yonginense sp. nov. (type strain HMD1001T=KCTC 22796T=CECT 7594T) and Flavobacterium myungsuense sp. nov. (type strain HMD1033T=KCTC 22825T=CECT 7649T) are proposed.
两株非运动性、革兰氏染色阴性、黄色色素的细菌菌株 HMD1001T 和 HMD1033T 从韩国一个中营养人工湖的水中分离得到。基于 16S rRNA 基因序列的系统发育树表明，这两株菌都可以归入黄杆菌属；菌株 HMD1001T 与 Flavobacterium fluvii H7T（96.8%序列相似性）、F. succinicans DSM 4002T（96.6%）和 F. hydatis DSM 2063T（96.6%）最为密切相关，而菌株 HMD1033T 与 Flavobacterium psychrolimnae LMG 2201T（96.2%）、F. segetis AT1048T（96.2%）和 F. weaverense AT1042T（96.2%）最为密切相关。菌株 HMD1001T 的主要脂肪酸为 iso-C15:0（21.5%）、summed feature 3（包含 C16:1ω6c 和/或 C16:1ω7c；18.0%）和 iso-C15:1 G（7.6%），而菌株 HMD1033T 的主要脂肪酸为 summed feature 3（23.8%）、iso-C15:0 3-OH（16.9%）、iso-C15:0（15.3%）和 anteiso-C15:0（12.1%）。菌株 HMD1001T 和 HMD1033T 的基因组 DNA G+C 含量分别为 35.9 和 32.2 mol%。基于系统发育和表型证据，菌株 HMD1001T 和 HMD1033T 代表黄杆菌属的两个新种，分别命名为永进黄杆菌（Flavobacterium yonginense sp. nov.，其模式菌株 HMD1001T=KCTC 22796T=CECT 7594T）和 Myungsu 黄杆菌（Flavobacterium myungsuense sp. nov.，其模式菌株 HMD1033T=KCTC 22825T=CECT 7649T）。
