NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):795-799. doi: 10.1099/ijs.0.031260-0. Epub 2011 May 13.
A non-motile and non-endospore-forming rod, strain NBRC 16403T, was isolated from the phyllosphere of a sedge (Carex sp.). 16S rRNA gene sequence analysis indicated that strain NBRC 16403T was closely related to Herbiconiux solani DSM 19813T (98.6 % 16S rRNA gene sequence similarity), Herbiconiux ginsengi wged11T (97.8 %) and Herbiconiux moechotypicola RB-62T (97.8 %). The peptidoglycan (B2γ type) contained D- and l-2,4-diaminobutyric acids, d-alanine, glycine and threo-3-hydroxyglutamic acid, which replaced glutamic acid almost completely. The predominant menaquinones were MK-10 and MK-11. The polar lipid pattern comprised diphosphatidylglycerol, phosphatidylglycerol, three glycolipids and minor amounts of other polar lipids. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0; no cyclohexyl-C17:0 was detected. The DNA G+C content was 71.0 mol%. The results of phylogenetic and DNA-DNA relatedness studies, along with phenotypic differences between strain NBRC 16403T and recognized members of the genus Herbiconiux, indicated that the isolate should be assigned to a novel species of the genus Herbiconiux, for which the name Herbiconiux flava sp. nov. is proposed. The type strain is NBRC 16403T (=VKM Ac-2058T).
一株非运动和非芽孢形成的杆状菌，NBRC 16403T 株，从莎草科植物（苔草属）的叶际中分离得到。16S rRNA 基因序列分析表明，NBRC 16403T 株与 Solani 属的 Herbiconiux solani DSM 19813T（98.6% 16S rRNA 基因序列相似性）、人参 Herbiconiux ginsengi wged11T（97.8%）和拟形 Herbiconiux moechotypicola RB-62T（97.8%）最为密切相关。肽聚糖（B2γ 型）含有 D-和 l-2,4-二氨基丁酸、d-丙氨酸、甘氨酸和苏氨酸-3-羟基谷氨酸，几乎完全取代了谷氨酸。主要的类异戊二烯醌为 MK-10 和 MK-11。极性脂类图谱包括双磷脂酰甘油、磷脂酰甘油、三种糖脂和少量其他极性脂类。主要脂肪酸为 anteiso-C15:0、iso-C16:0 和 anteiso-C17:0；未检测到环己基-C17:0。DNA G+C 含量为 71.0 mol%。基于系统发育和 DNA-DNA 同源性研究以及与 Herbiconiux 属已知种之间的表型差异，该分离株应被分配到 Herbiconiux 属的一个新种，提议将其命名为 Herbiconiux flava sp. nov.。模式菌株是 NBRC 16403T（=VKM Ac-2058T）。
