Senevirathna Dammika, Amuduwage Senani, Weerasingam Shirani, Jayasinghe Saroj, Fernandopulle Neil
Genetech Molecular Diagnostics and School of Gene Technology, University of Colombo, Colombo, Sri Lanka.
Asian J Transfus Sci. 2011 Jan;5(1):23-5. doi: 10.4103/0973-6247.75976.
Hepatitis C virus (HCV) is the etiological agent for the majority of cases of non-A, non-B hepatitis. As a blood-borne virus, HCV is widely recognized as a major causative agent of post-transfusion non-A, non-B hepatitis. The prevalence of HCV and the distribution of HCV genotypes in Sri Lanka in comparison with the rest of Asia are not well known.
The blood samples collected from healthy blood donors at the National Blood Transfusion Centre of Sri Lanka were screened to determine the prevalence and the genotypes of HCV among blood donors in Sri Lanka.
HCV antibodies were found in 53 of 4980 blood donors. However, of the 53 only 8 positive results were confirmed by Reverse Transcription-PCR, which suggests frequent false-positive results or viral clearance. The PCR positive samples were genotyped by DNA sequencing of the Core/E1 regions of HCV genome, and all the HCV viruses belonged to genotype 3, of which 7 were 3a and 1 was 3b.
HCV is relatively rare among blood donors in Sri Lanka and only genotype 3 was detected in the studied group.
丙型肝炎病毒（HCV）是大多数非甲非乙型肝炎病例的病原体。作为一种血源性病毒，HCV被广泛认为是输血后非甲非乙型肝炎的主要病原体。与亚洲其他地区相比，斯里兰卡HCV的流行情况和HCV基因型分布尚不清楚。
对从斯里兰卡国家输血中心健康献血者采集的血样进行筛查，以确定斯里兰卡献血者中HCV的流行情况和基因型。
在4980名献血者中，有53人检测出HCV抗体。然而，在这53人中，只有8个阳性结果通过逆转录聚合酶链反应得到确认，这表明存在频繁的假阳性结果或病毒清除情况。通过对HCV基因组的核心/E1区域进行DNA测序，对PCR阳性样本进行基因分型，所有HCV病毒均属于3型，其中7个为3a型，1个为3b型。
HCV在斯里兰卡献血者中相对少见，在所研究的群体中仅检测到3型。
