开发 16 个微卫星标记用于欧洲金龟子，Melolontha melolontha。

Development of 16 microsatellite markers for the European cockchafer, Melolontha melolontha.

机构信息

Agroscope Reckenholz-Tänikon Research Station, Molecular Ecology, Reckenholzstrasse 191, 8046 Zürich, Switzerland.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):158-60. doi: 10.1111/j.1471-8286.2007.01909.x.

DOI:10.1111/j.1471-8286.2007.01909.x

Abstract

Sixteen polymorphic microsatellite markers were isolated from the European cockchafer, Melolontha melolontha L., based on enriched genomic libraries. Allelic variability was assessed in a collection of 30 individuals obtained from a field in eastern Switzerland. The number of detected alleles ranged from three to 13 with a mean of 6.69 and average observed and expected heterozygosities were 0.52 and 0.66, respectively. The 16 highly polymorphic microsatellite markers represent a powerful molecular tool, which will allow for detailed population genetic analyses on this important pest insect.

摘要

从欧洲金龟子（Melolontha melolontha L.）的富集基因组文库中分离出 16 个多态性微卫星标记。在从瑞士东部一个田野中采集的 30 个个体的集合中评估了等位基因的变异性。检测到的等位基因数从 3 到 13 不等，平均值为 6.69，平均观察和预期杂合度分别为 0.52 和 0.66。这 16 个高度多态性微卫星标记代表了一种强大的分子工具，它将允许对这种重要的害虫昆虫进行详细的种群遗传分析。