从黄条纹绿鹃（Phyllastrephus flavostriatus）中分离和鉴定 10 个四核苷酸微卫星位点，并在四个密切相关的分类群中进行跨物种扩增。

Isolation and characterization of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul (Phyllastrephus flavostriatus) and cross-species amplification in four closely related taxa.

机构信息

DST/NRF Centre of Excellence at the Percy FitzPatrick Institute, Department of Botany and Zoology, Stellenbosch University, Private BagXI, Matieland 7602, South Africa, Museum of Vertebrate Zoology and Department of Integrative Biology, 3101 Valley Life Science Building, University of California, Berkeley, CA 94720, USA, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605, USA.

出版信息

Mol Ecol Resour. 2008 May;8(3):622-4. doi: 10.1111/j.1471-8286.2007.02023.x.

DOI:10.1111/j.1471-8286.2007.02023.x

PMID:21585851

Abstract

We describe the isolation of 10 tetranucleotide microsatellite loci from the yellow-streaked greenbul using an enrichment protocol. All loci were highly variable with the number of alleles ranging from 8 to 13, and observed heterozygosity ranging from 0.652 to 0.870. All loci were in Hardy-Weinberg equilibrium; however, loci Pfl12 and Pfl54 showed significant linkage disequilibrium. All 10 loci successfully amplified and were polymorphic in at least one of four related Phyllastrephus species. These loci should prove to be widely applicable to studies of phylogeography, hybridization and paternity in African greenbuls.

摘要

我们描述了使用富集方案从黄条纹绿霸鹟中分离出 10 个四核苷酸微卫星位点。所有位点都具有高度变异性，等位基因数从 8 到 13 不等，观察到的杂合度从 0.652 到 0.870 不等。所有位点均处于哈迪-温伯格平衡状态；然而，位点 Pfl12 和 Pfl54 表现出显著的连锁不平衡。在至少四个相关的 Phyllastrephus 物种中，所有 10 个位点都成功扩增并具有多态性。这些位点应该可以广泛应用于非洲绿霸鹟的系统地理学、杂交和亲子关系研究。