Pritzker Laboratory for Molecular Systematics and Evolution, Field Museum of Natural History, 1400 South Lake Shore Dr., Chicago, IL, 60605, USA.
Elasmo Project, P.O. Box 29588, Dubai, United Arab Emirates.
Mol Biol Rep. 2020 Apr;47(4):2669-2675. doi: 10.1007/s11033-020-05357-y. Epub 2020 Mar 4.
Microsatellites are useful in studies of population genetics, sibship, and parentage. Here, we screened for microsatellites from multiple elasmobranch genomic libraries using an enrichment protocol followed by sequencing on an Illumina platform. We concurrently screened five and then nine genomes and describe the number of potential loci from each respective round of sequencing. To validate the efficacy of the protocol, we developed and tested primers for the pelagic thresher shark, Alopias pelagicus. The method described here is a cost-effective protocol to increase the pool of potential useful loci and allows the concurrent screening of multiple libraries.
微卫星在种群遗传学、亲缘关系和亲子关系研究中很有用。在这里，我们使用富集方案从多个软骨鱼基因组文库中筛选微卫星，然后在 Illumina 平台上进行测序。我们同时筛选了 5 个和 9 个基因组，并描述了每一轮测序的潜在基因座数量。为了验证该方案的效果，我们为远洋长尾鲨（Alopias pelagicus）开发并测试了引物。这里描述的方法是一种经济有效的方案，可以增加潜在有用基因座的数量，并允许同时筛选多个文库。
