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从腺毛欧蓍草表达序列标签中鉴定和描述简单重复序列标记。

Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag.

机构信息

Institute for Applied Botany, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria, Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.

出版信息

Mol Ecol Resour. 2008 May;8(3):599-601. doi: 10.1111/j.1471-8286.2007.02059.x.

DOI:10.1111/j.1471-8286.2007.02059.x
PMID:21585844
Abstract

Oregano (Origanum vulgare) and marjoram (Origanum majorana) are two sensorial distinct spices within the genus Origanum (Lamiaceae). Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) of essential oil glands of O. vulgare. Thirteen EST-SSR loci were evaluated using 20 individual plants of O. vulgare and 19 plants of Origanum majorana. The number of alleles per locus ranged from one to four. All loci developed from O. vulgare successfully cross-amplified in O. majorana.

摘要

牛至(Origanum vulgare)和马郁兰(Origanum majorana)是唇形科牛至属中两种具有不同感官特性的香料。从牛至精油腺的表达序列标签(EST)中开发了简单序列重复(SSR)标记。使用 20 株牛至个体植物和 19 株马郁兰植物评估了 13 个 EST-SSR 基因座。每个基因座的等位基因数从 1 到 4 不等。从牛至属开发的所有基因座都能成功地在马郁兰中扩增。

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