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马铃薯中 NB-LRR 疾病抗性基因座的全基因组遗传图谱。

A genome-wide genetic map of NB-LRR disease resistance loci in potato.

机构信息

Laboratory of Nematology, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.

出版信息

Theor Appl Genet. 2011 Aug;123(3):493-508. doi: 10.1007/s00122-011-1602-z. Epub 2011 May 18.

DOI:10.1007/s00122-011-1602-z
PMID:21590328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3135832/
Abstract

Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato.

摘要

与所有植物一样，马铃薯进化出了一套由大量基因组成的监控系统，这些基因编码免疫受体，赋予其对病原体和害虫的抗性。这些所谓的抗性或 R 蛋白大多数属于超家族，含有核苷酸结合和富含亮氨酸重复结构域（NB-LRR）。在这里，我们使用保守的 NB 结构域的序列信息来研究马铃薯中 NB-LRR 抗性基因座的全基因组遗传分布。我们分析了使用二倍体马铃薯克隆 RH89-039-16（Solanum tuberosum ssp. tuberosum）的 BAC 文库的过滤杂交筛选对 288 个独特 BAC 克隆进行的序列选择，以及该 BAC 文库的物理图谱。这导致了 738 个部分和全长 NB-LRR 序列的鉴定。根据这些序列与已知抗性基因的同源性，将 280 个和 448 个序列分别归类为 TIR-NB-LRR（TNL）和 CC-NB-LRR（CNL）序列。遗传图谱显示存在 15 个 TNL 和 32 个 CNL 基因座。其中 36 个是新的，而 3 个 TNL 基因座和 8 个 CNL 基因座与先前鉴定的功能抗性基因具有同源性。遗传图谱补充了 68 个通用 CAPS 标记和 82 个文献中描述的疾病抗性性状基因座，为马铃薯的遗传研究和应用研究提供了极好的模板。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e0/3135832/4a0f9ab73440/122_2011_1602_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e0/3135832/f8443c04f2a6/122_2011_1602_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e0/3135832/4a0f9ab73440/122_2011_1602_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e0/3135832/f8443c04f2a6/122_2011_1602_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e0/3135832/4a0f9ab73440/122_2011_1602_Fig2_HTML.jpg

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