Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
J Infect Dis. 2011 Jun 1;203(11):1613-20. doi: 10.1093/infdis/jir112.
A pig ligated loop model was used to analyze the in vivo transcriptome response of Clostridium difficile. Bacterial RNA from the loops was retrieved at different times and was used for microarray analysis. Several virulence-associated genes and genes involved in sporulation cascade were differentially expressed (DE). In concordance with observed upregulation of toxin genes in microarray, enzyme-linked immunosorbent assay estimation of total toxin showed high amounts of toxin in the loops. Several genes that were absent in primary annotation of C. difficile 630 but annotated in a secondary annotation were found to be DE. Pathway comparison of DE genes in vitro and in vivo showed that when several pathways were expressed in all conditions, several of the C. difficile pathways were uniquely expressed only in vivo. The pathways observed to be modulated only in this study could be targets of new therapeutic agents against C. difficile infection.
我们使用结扎猪回肠模型来分析艰难梭菌的体内转录组反应。在不同时间从回肠环中提取细菌 RNA，并用于微阵列分析。一些毒力相关基因和参与孢子形成级联的基因表达存在差异（DE）。与微阵列观察到的毒素基因上调一致，酶联免疫吸附测定法估计总毒素显示在回肠环中有大量毒素。在艰难梭菌 630 的主要注释中不存在但在二级注释中注释的几个基因被发现存在 DE。体外和体内 DE 基因的途径比较表明，当几种途径在所有条件下表达时，几种艰难梭菌途径仅在体内独特表达。在这项研究中观察到的被调节的途径可能是针对艰难梭菌感染的新治疗剂的靶标。
