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重组痘苗病毒的瞬时显性选择

Transient dominant selection of recombinant vaccinia viruses.

作者信息

Falkner F G, Moss B

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

J Virol. 1990 Jun;64(6):3108-11. doi: 10.1128/JVI.64.6.3108-3111.1990.

DOI:10.1128/JVI.64.6.3108-3111.1990
PMID:2159565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249504/
Abstract

A general method for constructing and selecting recombinant vaccinia viruses with insertions, deletions, or mutations in any gene that is similar in principle to one originally devised for Saccharomyces cerevisiae (S. Scherer and R. W. Davis, Proc. Natl. Acad. Sci. USA 76:4951-4955, 1979) is described. The selectable marker used, Escherichia coli guanine phosphoribosyltransferase, is not retained within the final recombinant virus, and hence, this procedure may be used serially to introduce several foreign genes or to make multiple site-directed mutations.

摘要

本文描述了一种构建和筛选重组痘苗病毒的通用方法，该方法可用于在任何基因中进行插入、缺失或突变，其原理与最初为酿酒酵母设计的方法类似（S. 谢勒和R. W. 戴维斯，《美国国家科学院院刊》76:4951 - 4955, 1979）。所使用的选择标记是大肠杆菌鸟嘌呤磷酸核糖转移酶，它不会保留在最终的重组病毒中，因此，该程序可连续用于引入多个外源基因或进行多个位点定向突变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93ea/249504/f29a78bb16c7/jvirol00061-0670-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93ea/249504/391b57b3281e/jvirol00061-0669-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93ea/249504/f29a78bb16c7/jvirol00061-0670-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93ea/249504/391b57b3281e/jvirol00061-0669-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93ea/249504/f29a78bb16c7/jvirol00061-0670-a.jpg

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1
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Filamin B restricts vaccinia virus spread and is targeted by vaccinia virus protein C4.Filamin B 限制了牛痘病毒的传播，并且是牛痘病毒蛋白 C4 的作用靶点。
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本文引用的文献

1
Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters.酿酒酵母GAL基因簇的缺失分析。来自三个启动子的转录。
J Mol Biol. 1981 Oct 25;152(2):317-34. doi: 10.1016/0022-2836(81)90245-x.
2
Histone H2B subtypes are dispensable during the yeast cell cycle.组蛋白H2B亚型在酵母细胞周期中并非必需。
Cell. 1981 Aug;25(2):477-87. doi: 10.1016/0092-8674(81)90066-0.
3
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
Viruses. 2023 Aug 15;15(8):1742. doi: 10.3390/v15081742.
4
CRISPR-mediated rapid arming of poxvirus vectors enables facile generation of the novel immunotherapeutic STINGPOX.CRISPR 介导的快速武装痘病毒载体使新型免疫疗法 STINGPOX 的简便生成成为可能。
Front Immunol. 2023 Jan 13;13:1050250. doi: 10.3389/fimmu.2022.1050250. eCollection 2022.
5
Lumpy Skin Disease Virus with Four Knocked Out Genes Was Attenuated In Vivo and Protects Cattle from Infection.具有四个基因敲除的结节性皮肤病病毒在体内减毒，并能保护牛免受感染。
Vaccines (Basel). 2022 Oct 12;10(10):1705. doi: 10.3390/vaccines10101705.
6
Poxvirus Recombination.痘病毒重组
Pathogens. 2022 Aug 9;11(8):896. doi: 10.3390/pathogens11080896.
7
Genome Sequence of Atyrau-5BJN(IL18), a Recombinant Lumpy Skin Disease Virus with Knockout of Virulence Genes.阿特劳-5BJN（IL18）的基因组序列，一种具有毒力基因敲除的重组结节性皮肤病病毒
Microbiol Resour Announc. 2022 Jul 21;11(7):e0038022. doi: 10.1128/mra.00380-22. Epub 2022 Jun 21.
8
Dysregulation of Cellular VRK1, BAF, and Innate Immune Signaling by the Vaccinia Virus B12 Pseudokinase.痘苗病毒 B12 假激酶对细胞 VRK1、BAF 和固有免疫信号的调控失调。
J Virol. 2022 Jun 8;96(11):e0039822. doi: 10.1128/jvi.00398-22. Epub 2022 May 11.
9
Single Immunization with Recombinant ACAM2000 Vaccinia Viruses Expressing the Spike and the Nucleocapsid Proteins Protects Hamsters against SARS-CoV-2-Caused Clinical Disease.单次免疫表达刺突蛋白和核衣壳蛋白的重组 ACAM2000 痘苗病毒可保护仓鼠免受 SARS-CoV-2 引起的临床疾病。
J Virol. 2022 May 11;96(9):e0038922. doi: 10.1128/jvi.00389-22. Epub 2022 Apr 12.
10
Poxviruses and paramyxoviruses use a conserved mechanism of STAT1 antagonism to inhibit interferon signaling.痘病毒和副粘病毒利用一种保守的 STAT1 拮抗机制来抑制干扰素信号通路。
Cell Host Microbe. 2022 Mar 9;30(3):357-372.e11. doi: 10.1016/j.chom.2022.01.014. Epub 2022 Feb 18.
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6. doi: 10.1073/pnas.78.4.2072.
4
General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.表达外源基因的感染性痘苗病毒重组体的生产和选择通用方法。
J Virol. 1984 Mar;49(3):857-64. doi: 10.1128/JVI.49.3.857-864.1984.
5
Vaccinia virus: a selectable eukaryotic cloning and expression vector.痘苗病毒：一种可选择的真核克隆和表达载体。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9. doi: 10.1073/pnas.79.23.7415.
6
Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.痘病毒作为克隆载体的构建：将单纯疱疹病毒的胸苷激酶基因插入有感染性的痘苗病毒DNA中。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927-31. doi: 10.1073/pnas.79.16.4927.
7
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Proc Natl Acad Sci U S A. 1982 Feb;79(4):1210-4. doi: 10.1073/pnas.79.4.1210.
8
In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals.痘苗病毒基因启动子区域的体外诱变：串联早期和晚期调控信号的证据。
J Virol. 1985 Apr;54(1):30-7. doi: 10.1128/JVI.54.1.30-37.1985.
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Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.痘苗病毒表达载体：β-半乳糖苷酶的共表达可对重组病毒噬斑进行可视化筛选。
Mol Cell Biol. 1985 Dec;5(12):3403-9. doi: 10.1128/mcb.5.12.3403-3409.1985.
10
One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.痘苗病毒晚期基因5'侧翼序列的100个碱基对足以在时间上调控晚期转录。
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2096-100. doi: 10.1073/pnas.82.7.2096.