质谱分析加速了膜蛋白分析。
Mass spectrometry accelerates membrane protein analysis.
机构信息
Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
出版信息
Trends Biochem Sci. 2011 Jul;36(7):388-96. doi: 10.1016/j.tibs.2011.04.005. Epub 2011 May 26.
Abstract
Cellular membranes are composed of proteins and glyco- and phospholipids and play an indispensible role in maintaining cellular integrity and homeostasis, by physically restricting biochemical processes within cells and providing protection. Membrane proteins perform many essential functions, which include operating as transporters, adhesion-anchors, receptors, and enzymes. Recent advancements in proteomic mass spectrometry have resulted in substantial progress towards the determination of the plasma membrane (PM) proteome, resolution of membrane protein topology, establishment of numerous receptor protein complexes, identification of ligand-receptor pairs, and the elucidation of signaling networks originating at the PM. Here, we discuss the recent accelerated success of discovery-based proteomic pipelines for the establishment of a complete membrane proteome.
摘要
细胞膜由蛋白质、糖脂和磷脂组成，通过物理限制细胞内的生化过程并提供保护，在维持细胞完整性和动态平衡方面发挥着不可或缺的作用。膜蛋白具有许多重要的功能，包括作为转运蛋白、黏附锚、受体和酶发挥作用。蛋白质组学质谱分析技术的最新进展，使得人们在确定质膜（PM）蛋白质组、解析膜蛋白拓扑结构、建立众多受体蛋白复合物、鉴定配体-受体对以及阐明源自 PM 的信号网络方面取得了实质性的进展。在这里，我们讨论了基于发现的蛋白质组学管道最近取得的加速成功，以建立完整的膜蛋白质组。
相似文献
1
Mass spectrometry accelerates membrane protein analysis.质谱分析加速了膜蛋白分析。
Trends Biochem Sci. 2011 Jul;36(7):388-96. doi: 10.1016/j.tibs.2011.04.005. Epub 2011 May 26.
2
Advances in shotgun proteomics and the analysis of membrane proteomes. shotgun 蛋白质组学和膜蛋白质组分析的进展。
J Proteomics. 2010 Oct 10;73(11):2078-91. doi: 10.1016/j.jprot.2010.08.005. Epub 2010 Aug 23.
3
Mass spectrometry-based membrane proteomics in cancer biomarker discovery.基于质谱的癌症生物标志物发现中的膜蛋白质组学。
Expert Rev Mol Diagn. 2014 Jun;14(5):549-63. doi: 10.1586/14737159.2014.917965. Epub 2014 May 12.
4
Mass Spectrometry Untangles Plant Membrane Protein Signaling Networks.质谱技术解析植物膜蛋白信号网络。
Trends Plant Sci. 2020 Sep;25(9):930-944. doi: 10.1016/j.tplants.2020.03.013. Epub 2020 Apr 28.
5
Proteomic analysis of cell surface membrane proteins in leukemic cells.白血病细胞表面膜蛋白的蛋白质组学分析
Methods Mol Biol. 2007;370:135-46. doi: 10.1007/978-1-59745-353-0_11.
6
The application of mass spectrometry to membrane proteomics.质谱技术在膜蛋白质组学中的应用。
Nat Biotechnol. 2003 Mar;21(3):262-7. doi: 10.1038/nbt0303-262.
7
Proteomics and the dynamic plasma membrane: Quo Vadis?蛋白质组学与动态细胞膜：路在何方？
Proteomics. 2010 Nov;10(22):3997-4011. doi: 10.1002/pmic.201000312. Epub 2010 Nov 2.
8
An in vivo membrane density perturbation strategy for identification of liver sinusoidal surface proteome accessible from the vasculature.一种用于鉴定可从脉管系统获取的肝窦表面蛋白质组的体内膜密度扰动策略。
J Proteome Res. 2009 Jan;8(1):123-32. doi: 10.1021/pr8006683.
9
A stress test for mass spectrometry-based proteomics.基于质谱的蛋白质组学的压力测试。
Nat Methods. 2009 Jun;6(6):411-2. doi: 10.1038/nmeth.f.255.
10
Proteomic analysis of plasma membrane vesicles.质膜囊泡的蛋白质组学分析
Angew Chem Int Ed Engl. 2009;48(9):1656-9. doi: 10.1002/anie.200803898.
引用本文的文献
1
Fungal Coculture: Unlocking the Potential for Efficient Bioconversion of Lignocellulosic Biomass.真菌共培养：释放木质纤维素生物质高效生物转化的潜力。
J Fungi (Basel). 2025 Jun 17;11(6):458. doi: 10.3390/jof11060458.
2
Metal Organic Layers-Based Sample Preparation Method for Membrane Proteome Analysis.基于金属有机层的膜蛋白质组分析样品制备方法
Methods Mol Biol. 2025;2908:111-123. doi: 10.1007/978-1-0716-4434-8_8.
3
Investigating Anion Effects on Metal Ion Binding Interactions With Amyloid β Peptide by Ion Mobility Mass Spectrometry.通过离子淌度质谱法研究阴离子对金属离子与淀粉样β肽结合相互作用的影响。
J Mass Spectrom. 2024 Oct;59(10):e5090. doi: 10.1002/jms.5090.
4
Cell Surface Biotinylation Using Furan Cross-Linking Chemistry.使用呋喃交联化学进行细胞表面生物素化。
Methods Mol Biol. 2023;2718:11-21. doi: 10.1007/978-1-0716-3457-8_2.
5
Community- and genome-based evidence for a shaping influence of redox potential on bacterial protein evolution.基于群落和基因组的证据表明，氧化还原电位对细菌蛋白质进化具有塑造作用。
mSystems. 2023 Jun 29;8(3):e0001423. doi: 10.1128/msystems.00014-23. Epub 2023 Jun 8.
6
Emergence of mass spectrometry detergents for membrane proteomics.质谱去垢剂在膜蛋白质组学中的应用。
Anal Bioanal Chem. 2023 Jul;415(18):3897-3909. doi: 10.1007/s00216-023-04584-z. Epub 2023 Feb 18.
7
Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hub.通过纠正癫痫网络中枢的遗传缺陷，挽救 16p11.2 重复综合征小鼠模型的神经精神表型。
Nat Commun. 2023 Feb 17;14(1):825. doi: 10.1038/s41467-023-36087-x.
8
Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.植物中的逆行信号转导：聚焦 GUN 途径及其他方面的重要综述。
Plant Commun. 2023 Jan 9;4(1):100511. doi: 10.1016/j.xplc.2022.100511. Epub 2022 Dec 26.
9
GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction.GluR2Q 和 GluR2R AMPA 亚基不是 lypd2 相互作用的靶点。
PLoS One. 2022 Nov 28;17(11):e0278278. doi: 10.1371/journal.pone.0278278. eCollection 2022.
10
Lipid bilayer induces contraction of the denatured state ensemble of a helical-bundle membrane protein.脂质双层诱导螺旋束膜蛋白去折叠状态集合体的收缩。
Proc Natl Acad Sci U S A. 2022 Jan 4;119(1). doi: 10.1073/pnas.2109169119.
本文引用的文献
1
Systematic analysis of native membrane protein complexes in Escherichia coli.大肠杆菌天然膜蛋白复合物的系统分析。
J Proteome Res. 2011 Apr 1;10(4):1848-59. doi: 10.1021/pr101105c. Epub 2011 Feb 18.
2
Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability.使蛋白质表面的正电荷呈电中性会降低酰胺氢交换的速率，而不会改变其结构或增加其热稳定性。
J Am Chem Soc. 2010 Dec 15;132(49):17411-25. doi: 10.1021/ja9067035. Epub 2010 Nov 19.
3
Proteomics and transcriptomics characterization of bile stress response in probiotic Lactobacillus rhamnosus GG.益生菌鼠李糖乳杆菌 GG 应对胆液应激的蛋白质组学和转录组学特征。
Mol Cell Proteomics. 2011 Feb;10(2):M110.002741. doi: 10.1074/mcp.M110.002741. Epub 2010 Nov 15.
4
Temperature jump and fast photochemical oxidation probe submillisecond protein folding.温度跃变和快速光化学氧化探针亚毫秒级蛋白质折叠。
J Am Chem Soc. 2010 Nov 10;132(44):15502-4. doi: 10.1021/ja106518d.
5
Generating and navigating proteome maps using mass spectrometry.利用质谱技术生成和导航蛋白质组图谱。
Nat Rev Mol Cell Biol. 2010 Nov;11(11):789-801. doi: 10.1038/nrm2973. Epub 2010 Oct 14.
6
Comprehensive proteomic analysis of membrane proteins in Toxoplasma gondii.弓形虫膜蛋白的综合蛋白质组学分析。
Mol Cell Proteomics. 2011 Jan;10(1):M110.000745. doi: 10.1074/mcp.M110.000745. Epub 2010 Oct 10.
7
Site-directed mutagenesis combined with oxidative methionine labeling for probing structural transitions of a membrane protein by mass spectrometry.通过质谱法对膜蛋白结构转变进行探测的定点突变与氧化甲硫氨酸标记相结合。
J Am Soc Mass Spectrom. 2010 Nov;21(11):1947-56. doi: 10.1016/j.jasms.2010.08.004. Epub 2010 Aug 13.
8
Future directions of structural mass spectrometry using hydroxyl radical footprinting.利用羟基自由基足迹法进行结构质谱学的未来方向。
J Mass Spectrom. 2010 Dec;45(12):1373-82. doi: 10.1002/jms.1808.
9
Spatial organization of transmembrane receptor signalling.跨膜受体信号的空间组织。
EMBO J. 2010 Aug 18;29(16):2677-88. doi: 10.1038/emboj.2010.175.
10
Quantitative proteomics of the Cav2 channel nano-environments in the mammalian brain.哺乳动物脑内 Cav2 通道纳米环境的定量蛋白质组学。
Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):14950-7. doi: 10.1073/pnas.1005940107. Epub 2010 Jul 28.
Zotero 插件