Dzurba A, Michajlovski N, Ponec J, Okolicány J, Brlicová A, Lichardus B
Z Exp Chir. 1978;11(5):348-52.
Canine kidneys were preserved under hypothermia in Collins' standard solution and the contents of sodium, potassium, and Na+ and K+-ATPase in several parts of these kidneys were followed. Hypothermic preservation in combination with single perfusion by means of Collins' solution without thermic ischemia caused loss of sodium, increase of potassium, and decrease of the total osmotic cortico-papillary gradient of the kidney. No loss of Na+ and K+-ATPase activity occurred under these conditions. The determination of Na+ and K+-ATPase level in the renal tissue turns out to be a rational method to assess the vitality of an organ to be transplanted.
犬肾在低温条件下保存在柯林斯标准溶液中，并对这些肾脏几个部位的钠、钾含量以及钠钾 - ATP酶进行跟踪监测。低温保存结合柯林斯溶液单次灌注且无热缺血，导致肾脏钠流失、钾增加以及肾皮质 - 髓质总渗透梯度降低。在这些条件下，钠钾 - ATP酶活性没有损失。测定肾组织中钠钾 - ATP酶水平是评估待移植器官活力的一种合理方法。
