Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin People's Republic of China.
Genet Mol Biol. 2009 Jan;32(1):121-8. doi: 10.1590/S1415-47572009005000003. Epub 2009 Jan 10.
The nucleotide sequences of eight open reading frames (ORFs) located at the 5' end of the unique long region of the duck enteritis virus (DEV) Clone-03 strain were determined. The genes identified were designated UL1, UL2, UL3, UL4, UL5, UL6 and UL7 homologues of the herpes simplex virus 1 (HSV-1). The DEV UL3.5 located between UL3 and UL4 had no homologue in the HSV-1. The arrangement and transcription orientation of the eight genes were collinear with their homologues in the HSV-1. Phylogenetic trees were constructed based on the alignments of the deduced amino acids of eight proteins with their homologues in 12 alpha-herpesviruses. In the UL1, UL3, UL3.5, UL5 and UL7 proteins trees, the branches were more closely related to the genus Mardivirus. However, the UL2, UL4, and UL6 proteins phylogenetic trees indicated a large distance from Mardivirus, indicating that the DEV evolved differently from other viruses in the subfamily Alphaherpesvirinae and formed a single branch within this subfamily.
测定了鸭肠病毒（DEV）Clone-03 株独特长区 5'端 8 个开放阅读框（ORF）的核苷酸序列。鉴定的基因被命名为单纯疱疹病毒 1（HSV-1）的 UL1、UL2、UL3、UL4、UL5、UL6 和 UL7 同源物。位于 UL3 和 UL4 之间的 DEV UL3.5 在 HSV-1 中没有同源物。这 8 个基因的排列和转录方向与 HSV-1 中的同源物是一致的。基于 12 种α疱疹病毒中 8 种蛋白质推导的氨基酸序列与同源物的比对，构建了系统发育树。在 UL1、UL3、UL3.5、UL5 和 UL7 蛋白树上，分支与 Mardivirus 属更为密切相关。然而，UL2、UL4 和 UL6 蛋白的系统发育树表明与 Mardivirus 有很大的距离，表明 DEV 的进化与α疱疹病毒亚科中的其他病毒不同，并在该亚科内形成了一个单独的分支。
