Cavaş Tolga, Ergene-Gözükara Serap
Department of Biology, Faculty of Sciences & Letters, Mersin University, Ciftlikkoy 33342 Mersin, Turkey.
Mutat Res. 2003 Jul 8;538(1-2):81-91. doi: 10.1016/s1383-5718(03)00091-3.
In this study, cyto-genotoxic effects of a textile mill effluent on fish Oreochromis niloticus were investigated using the micronucleus (MN) test and methods to analyze interphase silver-stained nucleolar organizer regions (AgNORs). Fishes were exposed to three different concentrations of textile mill effluent (5, 10 and 20%(v/v)) for 3, 6 and 9 days. Cyclophosphamide (2mg/l) was used as a positive control. Micronucleus frequencies were examined in peripheral blood erythrocytes and gill cells. Nuclear abnormalities (NA) other than micronuclei such as binuclei, lobed nuclei, blebbed nuclei and notched nuclei were also evaluated in peripheral erythrocytes. Interphase AgNOR parameters were examined in epithelial cells obtained from the edge of caudal fins after 90 and 180min of exposure. As a result, dose-dependent increases in the frequencies of micronuclei and other NA in erythrocytes were observed. MN frequencies in gill cells also significantly increased, while the interphase AgNOR parameters in fin cells decreased, as a result of textile effluent and cylophosphamide treatments.
在本研究中，使用微核（MN）试验和分析间期银染核仁组织区（AgNORs）的方法，研究了纺织厂废水对尼罗罗非鱼的细胞遗传毒性作用。将鱼暴露于三种不同浓度的纺织厂废水（5%、10%和20%（v/v））中3天、6天和9天。环磷酰胺（2mg/l）用作阳性对照。在外周血红细胞和鳃细胞中检测微核频率。在外周红细胞中还评估了除微核以外的核异常（NA），如双核、分叶核、泡状核和缺口核。在暴露90分钟和180分钟后，从尾鳍边缘获得的上皮细胞中检测间期AgNOR参数。结果，观察到红细胞中微核和其他NA频率呈剂量依赖性增加。由于纺织废水和环磷酰胺处理，鳃细胞中的MN频率也显著增加，而鳍细胞中的间期AgNOR参数降低。
