Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Nanomedicine. 2011 Dec;7(6):965-74. doi: 10.1016/j.nano.2011.04.007. Epub 2011 May 19.
Functionalized fullerenes represent a new class of photosensitizer (PS) that is being investigated for photodynamic therapy (PDT) of various diseases, including cancer. We tested the hypothesis that fullerenes could be used to mediate PDT of intraperitoneal (IP) carcinomatosis in a mouse model. In humans this form of cancer responds poorly to standard treatment and manifests as a thin covering of tumor nodules on intestines and on other abdominal organs. We used a colon adenocarcinoma cell line (CT26) stably expressing luciferase to allow monitoring of IP tumor burden in BALB/c mice by noninvasive real-time optical imaging using a sensitive low-light camera. IP injection of a preparation of N-methylpyrrolidinium-fullerene formulated in Cremophor-EL micelles, followed by white-light illumination delivered through the peritoneal wall (after creation of a skin flap), produced a statistically significant reduction in bioluminescence and a survival advantage in mice.
This team of investigators report on functionalized fullerenes, to be used as photosensitizer for photodynamic therapy and demonstrate the efficacy of this method in an intraperitoneal carcinomatosis mouse model.
功能化富勒烯代表了一类新的光敏剂（PS），正在被研究用于各种疾病的光动力疗法（PDT），包括癌症。我们检验了这样一个假设，即富勒烯可用于介导腹腔内（IP）癌性腹水的 PDT，在小鼠模型中进行。在人类中，这种形式的癌症对标准治疗反应不佳，表现为肠道和其他腹部器官上的肿瘤结节的薄层覆盖。我们使用了一种稳定表达荧光素酶的结肠腺癌细胞系（CT26），允许通过使用灵敏的低光相机进行非侵入性实时光学成像来监测 BALB/c 小鼠的 IP 肿瘤负担。用 Cremophor-EL 胶束制成的 N-甲基吡咯烷富勒烯制剂进行 IP 注射，然后通过腹膜壁（在创建皮瓣后）进行白光照射，可在小鼠中产生统计学上显著降低的生物发光和生存优势。
本研究小组报告了功能化富勒烯，将其用作光动力疗法的光敏剂，并在腹腔内癌性腹水小鼠模型中证明了该方法的疗效。
