Gupta D K, Ing B L, Manahan F J, Zhou F Q, Yu A W, Nawab Z M, Rahman M A
Veterans Administration Hospital, Hines, Illinois.
Int J Artif Organs. 1990 Apr;13(4):228-30.
Neutrophils were isolated from canine blood and exposed to a conventional, acidic, lactate-containing peritoneal dialysis solution for 0, 2 and 4 minutes in one study and 0, 4, 7 and 10 minutes in another. Superoxide generation, expressed in nanomoles per million cells, was determined using a method based on the superoxide dismutase-inhibitable reduction of ferricytochrome c. Brief exposure of neutrophils to a conventional dialysis solution could significantly inhibit the generation of superoxide by neutrophils.
在一项研究中，从犬类血液中分离出中性粒细胞，并将其暴露于传统的、酸性的、含乳酸的腹膜透析液中0分钟、2分钟和4分钟；在另一项研究中暴露0分钟、4分钟、7分钟和10分钟。使用基于超氧化物歧化酶抑制的高铁细胞色素c还原法测定以每百万细胞纳摩尔数表示的超氧化物生成量。中性粒细胞短暂暴露于传统透析液中可显著抑制中性粒细胞产生超氧化物。
