Department of Biomedical Engineering, Reykjavik University, Iceland.
Adv Physiol Educ. 2011 Jun;35(2):188-96. doi: 10.1152/advan.00099.2010.
Recently, the zebrafish (Danio rerio) has been established as a key animal model in neuroscience. Behavioral, genetic, and immunohistochemical techniques have been used to describe the connectivity of diverse neural circuits. However, few studies have used zebrafish to understand the function of cerebral structures or to study neural circuits. Information about the techniques used to obtain a workable preparation is not readily available. Here, we describe a complete protocol for obtaining in vitro and in vivo zebrafish brain preparations. In addition, we performed extracellular recordings in the whole brain, brain slices, and immobilized nonanesthetized larval zebrafish to evaluate the viability of the tissue. Each type of preparation can be used to detect spontaneous activity, to determine patterns of activity in specific brain areas with unknown functions, or to assess the functional roles of different neuronal groups during brain development in zebrafish. The technique described offers a guide that will provide innovative and broad opportunities to beginner students and researchers who are interested in the functional analysis of neuronal activity, plasticity, and neural development in the zebrafish brain.
最近，斑马鱼（Danio rerio）已成为神经科学中的重要动物模型。行为学、遗传学和免疫组织化学技术已被用于描述不同神经回路的连接性。然而，很少有研究使用斑马鱼来了解大脑结构的功能或研究神经回路。有关获得可行的制备物的技术信息不容易获得。在这里，我们描述了一种完整的方案，用于获得体外和体内斑马鱼脑制备物。此外，我们在整个大脑、脑切片和固定的非麻醉幼鱼中进行了细胞外记录，以评估组织的活力。每种制备类型都可用于检测自发活动，确定具有未知功能的特定脑区的活动模式，或评估在斑马鱼脑发育过程中不同神经元群的功能作用。所描述的技术提供了一个指南，为有兴趣对斑马鱼大脑中的神经元活动、可塑性和神经发育进行功能分析的初学者学生和研究人员提供了创新和广泛的机会。
