Saint-Amant Louis, Drapeau Pierre
Department of Molecular Cellular and Developmental Biology, University of Michigan, 830 North University Ave., Ann Arbor, MI 48109-1048, USA.
Methods Cell Sci. 2003;25(1-2):59-64. doi: 10.1023/B:MICS.0000006896.02938.49.
We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these physiological techniques with the powerful genetic and molecular tools available in the zebrafish has the potential to increase our understanding of the complex interactions between genes and electrical activity during the development of the vertebrate nervous system.
我们描述了一种用于在体内斑马鱼标本中从已识别的胚胎脊髓中间神经元、运动神经元和感觉神经元获取膜片钳记录的制备方法。该制备方法用于研究脊髓回路初始发育过程中自发和触觉诱发电活动的空间和时间模式。这些生理学技术与斑马鱼中可用的强大遗传和分子工具相结合，有可能增进我们对脊椎动物神经系统发育过程中基因与电活动之间复杂相互作用的理解。
