Flemington E, Speck S H
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts.
J Virol. 1990 Sep;64(9):4549-52. doi: 10.1128/JVI.64.9.4549-4552.1990.
The Epstein-Barr virus BZLF1 gene product ZEBRA is a DNA-binding protein that is partially homologous to c-Fos, binds specifically to AP-1 sites, and can induce the lytic cycle in latently infected B lymphocytes. Induction of the viral lytic cycle can also be achieved by treatment with the phorbol ester 12-O-tetrade-canoylphorbol-13-acetate, a reagent which activates gene expression in part through AP-1 (Jun/Fos). In this article the interrelationship between ZEBRA and AP-1 is extended by the demonstration that ZEBRA can induce c-Fos expression through AP-1 and "AP-1-like" sites present in the c-fos promoter. Induction of c-Fos may be necessary for the expression of other viral lytic genes and perhaps cellular genes whose products are required for viral replication.
爱泼斯坦-巴尔病毒BZLF1基因产物ZEBRA是一种DNA结合蛋白，它与c-Fos部分同源，能特异性结合AP-1位点，并可在潜伏感染的B淋巴细胞中诱导裂解周期。用佛波酯12-O-十四烷酰佛波醇-13-乙酸酯处理也能诱导病毒裂解周期，该试剂部分通过AP-1（Jun/Fos）激活基因表达。在本文中，通过证明ZEBRA可通过c-fos启动子中存在的AP-1和“类AP-1”位点诱导c-Fos表达，扩展了ZEBRA与AP-1之间的相互关系。c-Fos的诱导对于其他病毒裂解基因以及可能对于病毒复制所需产物的细胞基因的表达可能是必要的。
