Divison of Enteric and Hepatitis viruses, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea.
Virol J. 2011 Jun 13;8:297. doi: 10.1186/1743-422X-8-297.
Previously, we explored the epidemic pattern and molecular characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006. The present study extended these observations to 2008 and 2009. In this study, enteroviruses showed similar seasonal prevalent pattern from summer to fall and age distribution to previous investigation. The most prevalent month was July: 42.9% in 2008 and 31.9% in 2009. The highest rate of enterovirus-positive samples occurred in children < 1-year-old-age. Enterovirus-positive samples were subjected to sequence determination of the VP1 region, which resolved the isolated enteroviruses into 10 types in 2008 (coxsackievirus A4, A16, B1, B3, echovirus 6, 7, 9, 11, 16, and 30) and 8 types in 2009 (coxsackievirus A2, A4, A5, A16, B1, B5, echovirus 11, and enterovirus 71). The most prevalent enterovirus serotype in 2008 and 2009 was echovirus 30 and coxsackievirus B1, respectively, whereas echovirus 18 and echovirus 5 were the most prevalent types in 2005 and 2006, respectively. Comparison of coxsackievirus B1 and B5 of prevalent enterovirus type in Korea in 2009 with reference strains of each same serotype were conducted to genetic analysis by a phylogenetic tree. The sequences of coxsackievirus B1 strains segregated into four distinct clusters (A, B, C, and D) with some temporal and regional sub-clustering. Most of Korean coxsackievirus B1 strains in 2008 and 2009 were in cluster D, while only "Kor08-CVB1-001CN" was cluster C. The coxsackievirus B5 strains segregated in five distinct genetic groups (clusters A-E) were supported by high bootstrap values. The Korean strains isolated in 2001 belonged to cluster D, whereas Korean strains isolated in 2005 and 2009 belonged to cluster E. Comparison of the VP1 amino acid sequences of the Korean coxsackievirus B5 isolates with reference strains revealed amino acid sequence substitutions at nine amino acid sequences (532, 562, 570, 571, 576-578, 582, 583, and 585).
此前，我们研究了韩国忠南地区 2005 年至 2006 年分离的肠道病毒的流行模式和分子特征。本研究将这些观察结果扩展到 2008 年和 2009 年。在本研究中，肠道病毒表现出与先前调查相似的季节性流行模式，从夏季到秋季，以及年龄分布。最常见的月份是 7 月：2008 年为 42.9%，2009 年为 31.9%。肠道病毒阳性样本率最高的是 <1 岁的儿童。对 VP1 区进行序列测定的肠道病毒阳性样本，将分离到的肠道病毒在 2008 年分为 10 种（柯萨奇病毒 A4、A16、B1、B3、埃可病毒 6、7、9、11、16 和 30）和 2009 年 8 种（柯萨奇病毒 A2、A4、A5、A16、B1、B5、埃可病毒 11 和肠道病毒 71）。2008 年和 2009 年最常见的肠道病毒血清型分别为埃可病毒 30 和柯萨奇病毒 B1，而 2005 年和 2006 年最常见的血清型分别为埃可病毒 18 和埃可病毒 5。对韩国 2009 年流行的肠道病毒类型 Coxsackievirus B1 和 B5 与各相同血清型的参考株进行遗传分析，通过系统进化树进行比较。柯萨奇病毒 B1 株分为四个不同的簇（A、B、C 和 D），具有一定的时间和区域亚簇。2008 年和 2009 年的大多数韩国柯萨奇病毒 B1 株属于 D 簇，而只有“Kor08-CVB1-001CN”属于 C 簇。柯萨奇病毒 B5 株分为五个不同的遗传群（簇 A-E），支持高自举值。2001 年分离的韩国株属于 D 簇，而 2005 年和 2009 年分离的韩国株属于 E 簇。与参考株相比，韩国柯萨奇病毒 B5 分离株的 VP1 氨基酸序列比较显示，在 9 个氨基酸序列（532、562、570、571、576-578、582、583 和 585）处存在氨基酸序列取代。
