Department of General Surgery, Affiliated Medical College Hospital, Qingdao University, Qingdao 266003, China.
Hepatobiliary Pancreat Dis Int. 2011 Jun;10(3):303-7. doi: 10.1016/s1499-3872(11)60050-7.
Many Chinese herbs, especially herbal injections, have been shown to have anti-tumor effects in recent years. However, since most reports focus on the clinical effectiveness of these herbs, their mechanisms of action are not well understood. In this study, we assessed apoptosis in the hepatocellular carcinoma (HCC) cell line HepG2 induced by an injectable extract from the seed of Coix lacryma-jobi (Semen coicis, SC), and monitored the expression of Bcl-2 and caspase-8.
Injectable SC was applied to HepG2 cells at different concentrations and the cells were collected 12, 24 and 48 hours later. 5-fluorouracil was used as a positive control group, and fluorescence-activated cell-sorting cytometry was used to measure the apoptosis rate of HepG2 cells and the expression of Bcl-2 and caspase-8 proteins.
SC induced apoptosis in HepG2 cells in a concentration- and time-dependent manner, and the expression of caspase-8 was elevated and prolonged. However, it did not significantly influence the expression of Bcl-2.
Injectable SC may induce apoptosis in HCC cells by regulating the expression of caspase-8.
近年来，许多中药，尤其是中药注射液，已被证明具有抗肿瘤作用。然而，由于大多数报告都集中在这些草药的临床疗效上，因此其作用机制尚不清楚。在这项研究中，我们评估了薏苡仁（Semen coicis，SC）注射液诱导肝癌（HCC）细胞系 HepG2 细胞凋亡的作用，并监测了 Bcl-2 和 caspase-8 的表达。
将不同浓度的可注射性 SC 应用于 HepG2 细胞，12、24 和 48 小时后收集细胞。5-氟尿嘧啶作为阳性对照组，通过流式细胞术检测 HepG2 细胞的凋亡率以及 Bcl-2 和 caspase-8 蛋白的表达。
SC 可浓度和时间依赖性地诱导 HepG2 细胞凋亡，且 caspase-8 的表达升高并延长。然而，它对 Bcl-2 的表达没有显著影响。
可注射性 SC 可能通过调节 caspase-8 的表达诱导 HCC 细胞凋亡。
