Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, 4726 beon-gil, Seodong-daero, Daedeok-myeon, Anseong-si, 17546, Gyeonggi-do, Republic of Korea.
Research Group of Food Safety, Korea Food Research Institute, 62, 1201 beon-gil, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
BMC Complement Altern Med. 2018 Mar 20;18(1):100. doi: 10.1186/s12906-018-2175-z.
It is well known that endoplasmic reticulum (ER) stress plays a huge role in development of metabolic diseases. Specially, ER stress-induced cellular dysfunction has a significant involvement in the pathogenesis of human chronic disorders. This study was designed to study to assess whether an ethanol extract of Coicis Semen (CSE) and coixol induces the ER stress in Chang liver cells.
Coicis Semen was mixed with 95% ethanol at a ratio of 1:10 (w/v) and freeze dried. Chang liver cells were seeded to 96-well plates and treated with or without CSE (100, 200, 300, 500, or 1000 μg/mL) or coixol (100, 200, 300, 500, 750, or 1000 μg/mL). cell viability was analyzed with MTT assay. Effects of CSE and coixol on expression of the genes for ER stress markers were determined with qRT-PCR and the expression of the protein levels of ER stress markers were determined with western blotting.
The concentration causing 50% inhibition (IC) for CSE and coixol was 250 and 350 μg/mL, respectively. The CSE and coixol increased the gene expression of BiP and CHOP in a dose-dependent manner. Furthermore, CSE and coixol dose-dependently increased the the expression of XBP1.
CSE or coixol may have cytotoxic effect to Chang liver cells and, may induce ER stress and stimulate the UPR via activation of the PERK and IRE1 pathways in normal liver cells.
内质网（ER）应激在代谢性疾病的发展中起着重要作用，这是众所周知的。特别是，ER 应激诱导的细胞功能障碍与人类慢性疾病的发病机制密切相关。本研究旨在评估薏苡仁乙醇提取物（CSE）和薏苡仁醇是否会引起Chang 肝细胞内质网应激。
薏苡仁与 95%乙醇按 1:10（w/v）的比例混合，冻干。将 Chang 肝细胞接种于 96 孔板，用或不用 CSE（100、200、300、500 或 1000μg/ml）或薏苡仁醇（100、200、300、500、750 或 1000μg/ml）处理。用 MTT 法分析细胞活力。用 qRT-PCR 测定 CSE 和薏苡仁醇对 ER 应激标志物基因表达的影响，用 Western blot 测定 ER 应激标志物蛋白水平的表达。
CSE 和薏苡仁醇的半数抑制浓度（IC）分别为 250 和 350μg/ml。CSE 和薏苡仁醇呈剂量依赖性增加 BiP 和 CHOP 的基因表达。此外，CSE 和薏苡仁醇剂量依赖性地增加 XBP1 的表达。
CSE 或薏苡仁醇可能对 Chang 肝细胞具有细胞毒性作用，可能通过激活 PERK 和 IRE1 途径诱导 ER 应激并刺激未折叠蛋白反应，从而刺激正常肝细胞中的 UPR。
