Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguanghuayuan Middle Road, Haidian District, Beijing 100097, China.
Mol Biol Rep. 2012 Mar;39(3):2893-900. doi: 10.1007/s11033-011-1049-y. Epub 2011 Jun 14.
The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) currently is one of the economically most destructive pest species of stone and pome fruits worldwide. Here we sequenced the complete mitochondrial genome of this pest. This genome is 15,776 bp long, with an A + T content of 81.24%, containing 37 typical animal mitochondrial genes and an A + T-rich region. All gene are arranged as hypothesized ancestral gene order of insects except for trnM, which was shuffled from 3' downstream of trnQ to 5' upstream of trnI. cox1 gene uses unusual CGA start codon, as that in all other sequenced lepidopteran mitochondrial genome. The secondary structures for the two rRNA genes were predicted. All helices typically present in insect mitochondrial rRNA genes are generated. A microsatellite sequence was inserted into the region of H2347 in rrnL in G. molesta and two other sequenced tortricid mitochondrial genomes, indicating that the insertion event in this helix might occurred anciently in family Tortricidae. All of the 22 typical animal tRNA genes have a typical cloverleaf structure except for trnS2, in which the D-stem pairings in the DHU arm are absent. An intergenic sequence is present between trnQ and nad2 as well as in other sequenced lepidopteran mitochondrial genomes, which was presumed to be a remnant of trnM gene and its boundary sequences after the duplication of trnM to the upstream of trnI in Lepidoptera. The A + T-rich region is 836 bp, containing six repeat sequences of "TTATTATTATTATTAAATA(G)TTT."
东方果实蝇，Grapholita molesta (Busck)（鳞翅目：卷蛾科）目前是世界范围内危害核果和梨果类水果最严重的经济害虫之一。本文对该害虫的线粒体基因组进行了测序。该基因组全长 15776bp，A+T 含量为 81.24%，包含 37 个典型动物线粒体基因和一个富含 A+T 的区域。除了 trnM 从 trnQ 的 3'下游移位到 trnI 的 5'上游外，所有基因均按昆虫假设的祖先基因顺序排列。cox1 基因使用不寻常的 CGA 起始密码子，与所有其他已测序鳞翅目线粒体基因组中的相同。预测了两个 rRNA 基因的二级结构。生成了昆虫线粒体 rRNA 基因中通常存在的所有螺旋。在 G. molesta 和另外两个已测序的卷蛾科线粒体基因组的 rrnL 中的 H2347 区插入了一个微卫星序列，表明该螺旋中的插入事件可能在卷蛾科中很早就发生了。除了 trnS2 之外，所有 22 个典型的动物 tRNA 基因都具有典型的三叶草结构，在 trnS2 中，DHU 臂的 D 茎配对不存在。trnQ 和 nad2 之间以及其他已测序鳞翅目线粒体基因组中存在一个基因间序列，推测这是 trnM 基因及其边界序列在 Lepidoptera 中复制到 trnI 上游后的残留物。富含 A+T 的区域为 836bp，包含六个“TTATTATTATTATTAAATA(G)TTT”重复序列。
