School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China.
Scientific Observing and Experimental Station of Crop Pest in Guiyang, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Ministry of Agriculture, Guiyang 550025, China.
Genes (Basel). 2023 Apr 8;14(4):883. doi: 10.3390/genes14040883.
is a flesh-feeding Diptera insect that adversely affects foodstuffs, such as dry-cured ham and cheese, and decaying human and animal carcasses. However, the unknown mitochondrial genome of can provide information on its genetic structure and phylogenetic position, which is of great significance to the research on its prevention and control. Therefore, we sequenced, annotated, and analyzed the previously unknown complete mitochondrial genome of . The complete mt genome of is a typical circular DNA, 15,785 bp in length, with a high A + T content of 76.6%. It contains 13 protein-coding genes (PCG), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 1 control region. Phylogenetic analysis of 25 Diptera species was conducted using Bayesian and maximum likelihood methods, and their divergence times were inferred. The comparison of the mt genomes from two morphologically similar insects and indicates a divergence time of 7.28 MYA between these species. The study provides a reference for understanding the forensic medicine, taxonomy, and genetics of .
是一种以肉为食的双翅目昆虫,会对干腌火腿、奶酪以及腐烂的人和动物尸体等食品造成不利影响。然而, 的未知线粒体基因组可以提供有关其遗传结构和系统发育位置的信息,这对于其防治的研究具有重要意义。因此,我们对 的未知完整线粒体基因组进行了测序、注释和分析。 的完整 mt 基因组是一个典型的圆形 DNA,长度为 15785bp,A+T 含量高达 76.6%。它包含 13 个蛋白质编码基因(PCG)、2 个核糖体 RNA(rRNA)基因、22 个转移 RNA(tRNA)基因和 1 个控制区。使用贝叶斯和最大似然法对 25 种双翅目物种进行了系统发育分析,并推断了它们的分歧时间。对两种形态相似的昆虫 和 的 mt 基因组进行比较,表明这两个物种之间的分化时间为 7.28MYA。该研究为了解 的法医学、分类学和遗传学提供了参考。