Faculty of Medicine, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Mycoses. 2011 Nov;54(6):e821-7. doi: 10.1111/j.1439-0507.2011.02036.x. Epub 2011 Jun 14.
Long-term continuous flow culture allows the investigation of dynamic biofilms under microaerophilic or aerobic conditions. We studied the biofilm formation and changes of susceptibility in 30 blood culture isolates (48 experiments) of different Candida species exposed to anidulafungin in 0.16 ml or 7.7 ml flow chambers. The flow rate (F) was adjusted to a very low rate of 1.3 ml h(-1) resulting in an exchange rate of up to 180 and 6.25 times chamber volumes per 24 hours in the small and large chambers, respectively. The results of culture at a very low flow rate were markedly different from cultures in micro well plates. Low flow rates may better mimic the in vivo situation and thus may be of higher relevance for the clinical setting. Under these conditions, a general resistance of fungal biofilms against anidulafungin cannot be confirmed. Strains of C. albicans and C. glabrata showed very uniform results whereas the C. parapsilosis group and C. lusitaniae varied from high susceptibility to resistance. Species differentiation of the C. parapsilosis group appears to be appropriate in clinical microbiological diagnostics. For the majority of the tested Candida species, anidualafungin was more effective than voriconazole. For the species C. lusitaniae and C. guilliermondii susceptibility testing should be considered prior to clinical use of echinocandin antifungals.
长期连续流动培养允许在微需氧或需氧条件下研究动态生物膜。我们研究了 30 株血培养分离株（48 个实验）在 0.16ml 或 7.7ml 流动室中暴露于安尼伐单抗时的生物膜形成和敏感性变化，这些分离株来自不同的念珠菌种。流速（F）被调整到非常低的速率 1.3ml/h，导致小室和大室中每 24 小时交换 chamber 体积高达 180 倍和 6.25 倍。在非常低流速下的培养结果与微孔板培养明显不同。低流速可能更好地模拟体内情况，因此可能与临床环境更相关。在这些条件下，不能确认真菌生物膜对安尼伐单抗的普遍耐药性。C. albicans 和 C. glabrata 菌株显示出非常一致的结果，而 C. parapsilosis 组和 C. lusitaniae 则从高敏感性到耐药性不等。C. parapsilosis 组的种分化在临床微生物学诊断中是合适的。对于大多数测试的念珠菌种，安尼伐单抗比伏立康唑更有效。对于 C. lusitaniae 和 C. guilliermondii 物种，在临床使用棘白菌素类抗真菌药物之前，应考虑进行药敏试验。
