Selow Marcela Lima Cardoso, Rymovicz Alinne Ulbrich Mores, Ribas Cristina Rauen, Saad Renata Simão, Rosa Rosimeire Takaki, Rosa Edvaldo Antonio Ribeiro
Xenobiotics Research Unit, The Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba, Zip 80250-901, Brazil.
Mycopathologia. 2015 Aug;180(1-2):27-33. doi: 10.1007/s11046-015-9889-y. Epub 2015 Apr 9.
A stainless steel paper-embedded biofilm reactor (PEBR) was developed for Candida spp. growth, permitting confluent distribution of nutrients by capillary diffusion through ordinary laboratory filter paper. Antibiogram disks were distributed along the filter paper rim, and the PEBR received 0.1 or 0.01 % crystal violet (CV) at 200 μL min(-1) and at 37 °C, for 48 h. CV was recovered from the disks and measured at 540 nm. Candida albicans SC5314 cells were applied onto antibiogram disks. The bioreactor was assembled, and YEPD broth was admitted (200 μL min(-1)) at 37 °C, for 72 h. Biofilm growth was estimated via the MTT reduction test. Controls were disks that received the same treatments, except for the fungus. The PEBR was considered high-throughput table, low-cost, and feasible to grow C. albicans biofilms.
开发了一种用于念珠菌属生长的不锈钢纸包埋生物膜反应器（PEBR），它通过普通实验室滤纸的毛细管扩散实现营养物质的汇合分布。抗菌药敏纸片沿滤纸边缘分布，PEBR在37℃下以200μL min⁻¹的流速接收0.1%或0.01%的结晶紫（CV），持续48小时。从纸片中回收CV并在540nm处测量。将白色念珠菌SC5314细胞接种到抗菌药敏纸片上。组装生物反应器，在37℃下以200μL min⁻¹的流速加入YEPD肉汤，持续72小时。通过MTT还原试验评估生物膜生长。对照组是接受相同处理但不含真菌的纸片。PEBR被认为是高通量、低成本且适合生长白色念珠菌生物膜的。
