Parisi Michael, Li Renhua, Oliver Brian
Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, 50 South Drive, Bethesda MD 20892, USA.
BMC Res Notes. 2011 Jun 15;4:198. doi: 10.1186/1756-0500-4-198.
D. melanogaster is increasingly used as a lipid metabolism model, but the D. melanogaster metabolome is not well studied. A number of studies strongly suggest that lipid metabolism is linked to sexual behavior and gametogenesis.
We determined the levels of 400 different lipids in the non-gonadal soma of D. melanogaster females and males. We found higher levels of saturated cholesterol esters and lysophosphatidylcholine in males, and higher levels of polyunsaturated cholesterol esters in females. We also determined the levels of these lipids in females and males without a germline to determine if the absence of gamete "sinks" for metabolic products, such as yolk and lipid deposits in eggs, altered somatic lipid profiles. We observed little change in lipid profiles between these samples.
Overall lipid compositions are similar between the sexes, although there are differences in saturation states of two lipid classes, where saturated fatty acids were male-biased and polyunsaturated fatty acids were female-biased. The presence of a germline did not significantly influence lipid profiles, raising the possibility that germline-dependent changes in metabolic gene expression patterns serve a homeostatic purpose.
黑腹果蝇越来越多地被用作脂质代谢模型，但对黑腹果蝇代谢组的研究并不充分。多项研究有力地表明，脂质代谢与性行为和配子发生有关。
我们测定了黑腹果蝇雌性和雄性非性腺体细胞中400种不同脂质的水平。我们发现雄性中饱和胆固醇酯和溶血磷脂酰胆碱的水平较高，而雌性中多不饱和胆固醇酯的水平较高。我们还测定了没有生殖系的雌性和雄性中这些脂质的水平，以确定卵黄和卵中脂质沉积等代谢产物的配子“汇”的缺失是否会改变体细胞脂质谱。我们观察到这些样本之间的脂质谱变化很小。
两性之间的总体脂质组成相似，尽管两类脂质的饱和状态存在差异，其中饱和脂肪酸在雄性中占优势，多不饱和脂肪酸在雌性中占优势。生殖系的存在并未显著影响脂质谱，这增加了代谢基因表达模式中依赖生殖系的变化具有稳态作用的可能性。
