Mu G F
Department of Microbiology, Faculty of Basic Medicine, Peking Union Medical College.
Zhonghua Liu Xing Bing Xue Za Zhi. 1990 Jun;11(3):154-5.
An pandemic of acute hemorrhagic conjunctivitis (AHC) caused by Coxsackievirus A 24 variant (CA 24v) occurred in China in 1988. Rapid diagnosis of AHC was investigated by immunofluorescence technique. The bacteria-free conjunctival swab samples were inoculated into HeLa cell monolayer. The Virus-specific antigens in infected cells were examined by indirect immunofluorescence test. Of 35 samples, 17 was identified as CA 24v. The results of isolation and typing could be obtained with in 5 days. Further, CA 24v antigen in infected conjunctival cells were examined by indirect immunofluorescence test. Of 48 smears of conjunctival cells, 39 was positive. Positive rate were 81.3%.
1988年中国发生了由柯萨奇病毒A24变种（CA24v）引起的急性出血性结膜炎（AHC）大流行。采用免疫荧光技术对AHC进行快速诊断。将无菌结膜拭子样本接种到单层HeLa细胞中。通过间接免疫荧光试验检测感染细胞中的病毒特异性抗原。35份样本中，17份被鉴定为CA24v。分离和分型结果可在5天内获得。此外，通过间接免疫荧光试验检测感染结膜细胞中的CA24v抗原。48份结膜细胞涂片中有39份呈阳性。阳性率为81.3%。
