School of Preventive Medicine, Third Military Medical University, Chongqing, China.
Neurol Sci. 2011 Aug;32(4):641-51. doi: 10.1007/s10072-011-0608-2. Epub 2011 Jun 16.
The efficacy of granulocyte colony-stimulating factor (G-CSF) in mobilizing mesenchymal stem cells (MSCs) into peripheral blood (PB) and the ability of PB-MSCs incorporated into injured brain were tested. Colony forming, cell phenotype and differentiation potential of mouse MSCs mobilized by G-CSF (40 μg/kg) were evaluated. Mortality and pathological changes in mice with serious craniocerebral trauma plus G-CSF treatment (40 μg/kg) were investigated. Bone marrow (BM) cells derived from GFP mice were fractionated into MSCs, hematopoietic stem cells (HSCs), and non-MSC/HSCs using magnetic beads and adherent culture. The resultant cell populations were transplanted into injured mice. The in vivo integration and differentiation of the transplanted cells were detected immunocytochemically. The expression of SDF-1 in injured area of brain was tested by Western blot. G-CSF was able to mobilize MSCs into PB (fourfold increase). PB-MSCs possessed similar characteristics as BM-MSCs in terms of colony formation, the expression pattern of CD73, 44, 90, 106, 31 and 45, and multipotential of differentiation. Accumulative total mice mortality was lower in TG group (5/14) than that in T group (7/14). It was MSCs, not HSCs or non-MSC/HSC cells integrated into the damaged cerebral tissue and differentiated into cells expressing neural markers. Increased SDF-1 expression in injured area of brain was confirmed, which could facilitate the homing of MSCs to brain. G-CSF can mobilize MSCs into PB and MSCs in PB can integrate into injured cerebral tissue and transdifferentiated into neural cells and may benefit the repair of trauma.
粒细胞集落刺激因子（G-CSF）动员间充质干细胞（MSCs）进入外周血（PB）的功效以及整合入损伤脑内的 PB-MSCs 的能力已被检测。对 G-CSF（40μg/kg）动员的小鼠 MSCs 的集落形成、细胞表型和分化潜能进行了评估。通过 G-CSF（40μg/kg）治疗严重颅脑创伤小鼠的死亡率和病理变化进行了研究。使用磁珠和贴壁培养将源自 GFP 小鼠的骨髓（BM）细胞分离为 MSCs、造血干细胞（HSCs）和非 MSC/HSCs。将得到的细胞群移植到损伤的小鼠中。通过免疫细胞化学检测移植细胞的体内整合和分化。通过 Western blot 检测损伤脑区 SDF-1 的表达。G-CSF 能够将 MSCs 动员到 PB（增加四倍）。PB-MSCs 在集落形成、CD73、44、90、106、31 和 45 的表达模式以及多向分化潜能方面与 BM-MSCs 具有相似的特征。TG 组（5/14）的累积总死亡率低于 T 组（7/14）。是 MSCs，而不是 HSCs 或非 MSC/HSC 细胞整合到受损的脑组织中并分化为表达神经标记的细胞。证实了损伤脑区 SDF-1 表达增加，这有助于 MSC 归巢到脑。G-CSF 可以动员 MSCs 进入 PB，PB 中的 MSCs 可以整合到损伤的脑组织中并转分化为神经细胞，可能有益于创伤修复。
