School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province, China.
J Appl Microbiol. 2011 Sep;111(3):547-58. doi: 10.1111/j.1365-2672.2011.05080.x. Epub 2011 Jul 6.
To isolate and characterize the novel bioflocculant-producing bacteria, to optimize the bioflocculant production and to evaluate its potential applications.
Klebsiella pneumoniae strain NY1, a bacterium that produces a novel bioflocculant (MNXY1), was selected on the chemically defined media. It was classified according to the 16S rRNA gene sequence, morphological and microscopic characteristics. MNXY1 was characterized to contain 26% protein and 66% total sugar. The constituent sugar monomers of MNXY1, revealed by NMR analysis, are glucose, galactose and quinovose. Favourable culture conditions for MNXY1 production were determined. Strain NY1 produces a high level (14.9 g l(-1)) of MNXY1. MNXY1 is thermostable and tolerant to the extreme pH. It precipitated 54% of cyanobacteria from laboratory culture and 72% of the total suspended solids from raw wastewater.
Strain NY1 was identified to produce a novel bioflocculant MNXY1. The outstanding performance of MNXY1 in practical applications and its availability in copious amounts make it attractive for further investigation and development for industrial scale applications.
This is first report for the identification of a quinovose-containing bioflocculant and application of a protein-polysaccharide complex bioflocculant in precipitation of cyanobacteria. These findings suggest that MNXY1 holds great potential for use in management of harmful algae and city wastewater treatment.
分离和鉴定新型产絮菌，优化生物絮凝剂的生产，并评估其潜在应用。
选择一株能产生新型生物絮凝剂（MNXY1）的肺炎克雷伯菌（Klebsiella pneumoniae）菌株 NY1，在化学成分确定的培养基上进行筛选。根据 16S rRNA 基因序列、形态学和显微镜特征对其进行分类。MNXY1 被鉴定为含有 26%的蛋白质和 66%的总糖。通过 NMR 分析，MNXY1 的组成糖单体为葡萄糖、半乳糖和奎诺糖。确定了 MNXY1 生产的最佳培养条件。NY1 菌株可产生高水平的 MNXY1（14.9 g l(-1)）。MNXY1 具有热稳定性和耐极端 pH 值的特性。它可以从实验室培养的蓝藻中沉淀 54%，从原废水的总悬浮固体中沉淀 72%。
鉴定出 NY1 菌株能产生新型生物絮凝剂 MNXY1。MNXY1 在实际应用中表现出色，且易于大量生产，这使其成为进一步研究和开发工业规模应用的有吸引力的选择。
这是首次报道鉴定出含有奎诺糖的生物絮凝剂，并应用蛋白质-多糖复合物生物絮凝剂来沉淀蓝藻。这些发现表明，MNXY1 在有害藻类和城市污水处理管理方面具有很大的应用潜力。
