Erdem Arzu Pinar, Sepet Elif, Avshalom Tam, Gutkin Vitaly, Steinberg Doron
Istanbul University, Faculty of Dentistry, Department of Pedodontics, Capa/Istanbul 34093 Turkey.
Am J Dent. 2011 Apr;24(2):119-23.
(1) To determine the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and acidulated phosphate fluoride (APF) on S. mutans viability, (2) to observe their effects on biofilm structure, and (3) to examine the element content of the hydroxyapatite (HA) surfaces after exposure to CPP-ACP and APF.
HA discs were coated with: CPP-ACP (GC Tooth-Mousse), APF, CPP-ACP+APF (1/1). Uncoated HA discs were used as control. Following application of the materials, the discs were immersed in human saliva and incubated with S. mutans ATCC (27315) for 24 hours. Growth of bacteria on the discs was evaluated by microbial culturing methods. The structure of the biofilm was examined with confocal laser scanning microscope (CLSM). The change in element content of HA surfaces (without biofilm) was evaluated with energy-dispersive x-ray spectroscopy (EDS). The values were statistically analyzed using Kruskal-Wallis and Dunn's test.
The total number of bacteria of APF and CPP-ACP+APF applied groups were found significantly lower than the control group (P< 0.05). All specimens showed similar microbial colonization structure. No statistically significant differences were observed in O, F, Na, P, Ca content on HA surfaces after exposure to the tested agents, although fluoride concentration of the APF treated HA surfaces were increased compared to CPP-ACP, CPP-ACP +APF.
（1）确定酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）和酸化磷酸氟（APF）对变形链球菌生存能力的影响；（2）观察它们对生物膜结构的影响；（3）检测暴露于CPP - ACP和APF后羟基磷灰石（HA）表面的元素含量。
将HA圆盘分别用以下物质包被：CPP - ACP（GC护齿泡沫）、APF、CPP - ACP + APF（1/1）。未包被的HA圆盘用作对照。应用这些材料后，将圆盘浸入人唾液中，并与变形链球菌ATCC（27315）一起孵育24小时。通过微生物培养方法评估圆盘上细菌的生长情况。用共聚焦激光扫描显微镜（CLSM）检查生物膜的结构。用能量色散X射线光谱仪（EDS）评估HA表面（无生物膜）的元素含量变化。使用Kruskal - Wallis检验和Dunn检验对数据进行统计学分析。
发现应用APF和CPP - ACP + APF组的细菌总数显著低于对照组（P < 0.05）。所有标本均显示出相似的微生物定植结构。暴露于受试剂后，HA表面的O、F、Na、P、Ca含量未观察到统计学上的显著差异，尽管与CPP - ACP、CPP - ACP + APF相比，经APF处理的HA表面氟浓度有所增加。
