School of Graduate Dentistry, Technion-Israel Institute of Technology, P.O. Box 9602, Haifa, 31096, Israel.
J Dent Res. 2011 Sep;90(9):1136-9. doi: 10.1177/0022034511413283. Epub 2011 Jun 23.
The fast and efficient transportation among continents will continue to play a role in the spread of airborne pandemics. The objective of this study was to detect H1N1 virus in the saliva of individuals who visited the emergency department and were diagnosed as having H1N1 influenza. Nasopharyngeal swabs and saliva samples from those who presented to the emergency department with flu-like symptoms were sent to the laboratory. RNA was extracted from both samples. Real-time RT-PCR tests were performed, and the saliva and nasopharyngeal swab tests were compared. Samples were drawn from 26 individuals. A positive nasopharyngeal swab test and salivary test was found in 14 persons, and negative tests were found in 12 persons. Saliva sampling for H1N1 has excellent predictive value, is highly accurate and reliable, and is more convenient than the nasopharyngeal swab. Clinical trial with the Helsinki Committee at Rambam Health Care Campus, registration number 036309-RMB.
在大陆间快速高效的交通将继续在空气传播的大流行传播中发挥作用。本研究的目的是检测在急诊就诊并被诊断为感染 H1N1 流感的个体的唾液中的 H1N1 病毒。对有流感样症状就诊于急诊的患者采集鼻咽拭子和唾液样本，并送往实验室。从两个样本中提取 RNA，进行实时 RT-PCR 检测，并比较唾液和鼻咽拭子的检测结果。共抽取 26 例样本。14 例患者的鼻咽拭子和唾液检测均为阳性，12 例为阴性。唾液取样检测 H1N1 具有极好的预测价值，高度准确可靠，比鼻咽拭子更方便。在海法 Rambam 医疗园区赫尔辛基委员会的临床试验，注册号 036309-RMB。
