Howard Hughes Medical Institute (HHMI), Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3204, USA.
Science. 2011 Jul 15;333(6040):307. doi: 10.1126/science.1207773. Epub 2011 Jun 23.
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
进化研究对于剖析各种生物过程是必要的，但由于大多数具有测序基因组的生物缺乏反向遗传方法，因此受到限制。我们使用锌指核酸酶 (ZFNs) 和转录激活因子样效应物核酸酶 (TALENs) 建立了一种广泛适用的策略，用于靶向敲除分化线虫物种中的内源性基因和顺式作用调控元件。
