Sse8387I，一种新型II型限制性内切酶，可识别八核苷酸序列5'-CCTGCAGG-3'。

Kotani H, Nomura Y, Kawashima Y, Sagawa H, Takagi M, Kita A, Ito H, Kato I

Bioproducts Development and Research Center, Takara Shuzo Co., Ltd., Shiga, Japan.

Nucleic Acids Res. 1990 Oct 11;18(19):5637-40. doi: 10.1093/nar/18.19.5637.

A type II restriction endonuclease designated Sse8387I was partially purified from Streptomyces sp. 8387. This enzyme cleaved adenovirus 2 DNA at three sites, lambda phage DNA at five sites, and pUC18 and M13mp18 RF DNA at one site each, but did not cleave the DNAs from pBR322, SV40, or phi X174. Sse8387I recognized the octanucleotide sequence 5'-CCTGCA decreases GG-3', cleaving where shown by the arrow. Sse8387I is the first restriction endonuclease to be reported that recognizes an octanucleotide sequence consisting of all four nucleotides, G, A, T, and C. The frequency of occurrence of Sse8387I sites within sequenced regions of primate genomes was 2.4 times that of NotI sites.

一种名为Sse8387I的II型限制性内切酶从链霉菌属8387菌株中部分纯化出来。这种酶在三个位点切割腺病毒2 DNA，在五个位点切割λ噬菌体DNA，在pUC18和M13mp18 RF DNA各一个位点进行切割，但不切割来自pBR322、SV40或φX174的DNA。Sse8387I识别八核苷酸序列5'-CCTGCA↓GG-3'，在箭头所示位置切割。Sse8387I是第一个被报道识别由所有四种核苷酸G、A、T和C组成的八核苷酸序列的限制性内切酶。灵长类基因组测序区域内Sse8387I位点的出现频率是NotI位点的2.4倍。