一种来自流苏链霉菌的具有八个核苷酸特异性的II型限制性内切酶。
A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus.
作者信息
Qiang B Q, Schildkraut I
出版信息
Nucleic Acids Res. 1984 Jun 11;12(11):4507-16. doi: 10.1093/nar/12.11.4507.
Abstract
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.
摘要
一种新的位点特异性内切核酸酶Sfi I已从纤维链霉菌中分离出来。这是关于一种II型限制性内切核酸酶的首次报道，其识别特异性需要八个核苷酸。Sfi I对称切割序列GGCCNNNN / NGGCC，产生一个三碱基的3'端延伸。
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