斯瓦I酶，一种来自沃纳氏葡萄球菌的独特限制性内切酶，其识别序列为5'-ATTTAAAT-3'。
SwaI, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'.
作者信息
Lechner M, Frey B, Laue F, Anton-Botella J, Smith C L, Ankenbauer W, Schmitz G G
机构信息
Boehringer Mannheim GmbH, Biochemical Research Center, Penzberg, Germany.
出版信息
Nucleic Acids Res. 1992 May 11;20(9):2293-6. doi: 10.1093/nar/20.9.2293.
A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
一种名为SwaI的新型II类限制性内切酶是从沃氏葡萄球菌中纯化出来的。这种酶分别在腺病毒2 DNA、SV40 DNA和M13mp7的一个位点切割，但不切割λ噬菌体、PhiX174、pBR322或pBR328 DNA。SwaI识别八核苷酸序列5'-ATTTAAAT-3'，在识别序列的中心进行切割，产生平端DNA片段。SwaI被用于消化来自各种微生物和人类细胞的染色体DNA。
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