Institution of Healthy and Toxicology, Beijing Centers for Disease Prevention and Control, Beijing, China.
Chin J Integr Med. 2011 Oct;17(10):764-9. doi: 10.1007/s11655-011-0642-1. Epub 2011 Jun 29.
To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (A.E.), a Chinese medicinal herb, against coxsackievirus B3 (CVB3).
The antiviral effects of A.E. against CVB3 in vitro (primarily cultured myocardial cells) and in vivo (BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.
A.E. exhibited obvious antiviral: effects in vivo, and serum samples obtained from the rats with oral administration of A.E. (10 μg/mL, 5 μg/mL), reduced the virus titers in the infected myocardial cells (3.00±0.70, 3.55±0.52, P<0.01). Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% (P<0.01) in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P<0.01). Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.
Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo.
研究鸡血藤水提物（A.E.）对柯萨奇病毒 B3（CVB3）的抗病毒作用。
测定 A.E. 对 CVB3 的体外（主要培养心肌细胞）和体内（BALB/c 小鼠）抗病毒作用。体外实验还采用血清药理学方法。通过体内小鼠 RT-PCR 比较 A.E. 抑制 CVB3 mRNA 表达的作用。
A.E. 在体内具有明显的抗病毒作用，口服 A.E.（10μg/mL、5μg/mL）的大鼠血清样本降低了感染心肌细胞中的病毒滴度（3.00±0.70、3.55±0.52，P<0.01）。同时，A.E. 显著抑制 CVB3 引起的病毒性心肌炎，A.E. 剂量为 50mg/kg 和 100mg/kg 时，小鼠 15 天死亡率分别降至 40%和 45%（P<0.01），30 天死亡率分别降至 45%和 50%（P<0.01）。此外，A.E. 显著抑制柯萨奇病毒 B3 的 mRNA 表达。
鸡血藤水提物（A.E.）对 CVB3 具有体内外抑制作用。
