Dipartimento di Sanità Pubblica e Malattie Infettive, Università La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Clin Oral Investig. 2012 Jun;16(3):987-95. doi: 10.1007/s00784-011-0585-0. Epub 2011 Jul 1.
This study was aimed at looking into the microbiological/inflammatory parameters predicting the periodontal success/failure of fixed prostheses. Microbiological and inflammatory patterns were studied at 102 sites having metaloceramic crowns in place from 3 to 6 years and divided in healthy sites (HS), gingivitis affected (MG), and periodontitis affected (PB). Total bacterial flora and selected indicator species in subgingival plaque were quantified by quantitative real-time PCR. The concentrations of IL-1β, IL-6, and TNF-α were determined in gingival crevicular fluid (GCF) by enzyme-linked immunosorbent assays. The experimental sites showed no significant difference with respect to the age and gender of the patients and to the position of the crown margins. Poor marginal adaptation was significantly higher in MG and PB. The total amounts of bacteria per probing depth showed no significant differences among the three groups and their controls, while both MG and PB sites showed altered patterns in the distribution of specific bacteria. Both MG and PB sites showed significantly higher levels of inflammatory cytokines in GCF. The control teeth of PB subjects showed significantly higher levels of IL-1β as compared to other control sites. Data confirm that the application of metaloceramic crowns is a factor of risk for the development of gingival/periodontal inflammation. This risk is possibly associated with microbiological and host factors that predispose to the onset of periodontal alterations at sites reconstructed with metaloceramic crowns. These factors, once their role is confirmed by longitudinal studies, could be used to set up rapid tests to early predict the onset of periodontal disease at reconstructed sites.
本研究旨在探讨预测固定修复体牙周成功/失败的微生物/炎症参数。对 102 个部位的微生物和炎症模式进行了研究，这些部位在 3 至 6 年内有金属陶瓷冠，分为健康部位（HS）、牙龈炎影响部位（MG）和牙周炎影响部位（PB）。通过定量实时 PCR 定量龈下菌斑中的总细菌菌群和选定的指示菌。通过酶联免疫吸附试验测定龈沟液（GCF）中 IL-1β、IL-6 和 TNF-α的浓度。实验部位在患者的年龄和性别以及冠缘位置方面没有显著差异。MG 和 PB 中边缘适应性差的比例明显更高。三组及其对照组的每个探诊深度的细菌总量无显著差异，而 MG 和 PB 部位的特定细菌分布模式发生改变。MG 和 PB 部位的 GCF 中炎症细胞因子水平均显著升高。与其他对照组相比，PB 受试者的对照牙齿的 IL-1β 水平明显更高。数据证实金属陶瓷冠的应用是牙龈/牙周炎发展的危险因素。这种风险可能与微生物和宿主因素有关，这些因素易导致金属陶瓷冠修复部位发生牙周改变。这些因素一旦通过纵向研究得到证实，就可以用于建立快速测试来早期预测重建部位牙周病的发生。
