采用斑点杂交免疫酶法快速检测尿样中的巨细胞病毒DNA。
Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay.
作者信息
Musiani M, Zerbini M, Gentilomi G, Gallinella G, Venturoli S, Gibellini D, La Placa M
机构信息
Institute of Microbiology, University of Bologna, Italy.
出版信息
J Clin Microbiol. 1990 Sep;28(9):2101-3. doi: 10.1128/jcm.28.9.2101-2103.1990.
Abstract
A dot blot hybridization immunoenzymatic assay for the rapid detection of cytomegalovirus DNA in urine samples was developed by using a digoxigenin-labeled probe which was immunoenzymatically visualized by antidigoxigenin Fab fragments labeled with alkaline phosphatase. A total of 516 urine samples from different groups of subjects were analyzed, and the hybridization assay was able to yield results within 24 h. The results obtained were compared with results for detection of cytomegalovirus antigens in infected cell cultures.
摘要
利用地高辛标记的探针，通过用碱性磷酸酶标记的抗地高辛Fab片段进行免疫酶可视化，开发了一种用于快速检测尿液样本中巨细胞病毒DNA的斑点杂交免疫酶测定法。共分析了来自不同受试者组的516份尿液样本，该杂交测定法能够在24小时内得出结果。将获得的结果与在感染细胞培养物中检测巨细胞病毒抗原的结果进行了比较。
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