Bundeswehr Institute of Microbiology, Munich, Germany.
Virol J. 2011 Jul 5;8:339. doi: 10.1186/1743-422X-8-339.
Approximately 60% of emerging viruses are of zoonotic origin, with three-fourths derived from wild animals. Many of these zoonotic diseases are transmitted by rodents with important information about their reservoir dynamics and pathogenesis missing. One main reason for the gap in our knowledge is the lack of adequate cell culture systems as models for the investigation of rodent-borne (robo) viruses in vitro. Therefore we established and characterized a new cell line, BVK168, using the kidney of a bank vole, Myodes glareolus, the most abundant member of the Arvicolinae trapped in Germany.
BVK168 proved to be of epithelial morphology expressing tight junctions as well as adherence junction proteins. The BVK168 cells were analyzed for their infectability by several arbo- and robo-viruses: Vesicular stomatitis virus, vaccinia virus, cowpox virus, Sindbis virus, Pixuna virus, Usutu virus, Inkoo virus, Puumalavirus, and Borna disease virus (BDV). The cell line was susceptible for all tested viruses, and most interestingly also for the difficult to propagate BDV.
In conclusion, the newly established cell line from wildlife rodents seems to be an excellent tool for the isolation and characterization of new rodent-associated viruses and may be used as in vitro-model to study properties and pathogenesis of these agents.
大约 60%的新兴病毒具有动物源性，其中四分之三来源于野生动物。这些动物源性疾病中有许多是通过啮齿动物传播的，而我们对其宿主动态和发病机制的了解却很有限。造成我们知识空白的一个主要原因是缺乏足够的细胞培养系统作为体外研究啮齿动物传播（robo）病毒的模型。因此，我们使用德国捕获的最丰富的田鼠科成员——林姬鼠的肾脏，建立并鉴定了一种新的细胞系 BVK168。
BVK168 表现出上皮形态，表达紧密连接和黏附连接蛋白。分析了 BVK168 细胞对几种 arbovirus 和 robo-viruses 的感染性：水疱性口炎病毒、牛痘病毒、牛痘病毒、辛德毕斯病毒、皮库纳病毒、乌苏图病毒、因科病毒、普马拉病毒和博纳病病毒（BDV）。该细胞系对所有测试的病毒均易感，最有趣的是对难以繁殖的 BDV 也是如此。
总之，从野生动物啮齿动物中建立的新细胞系似乎是分离和鉴定新的啮齿动物相关病毒的极好工具，并且可以用作体外模型来研究这些病毒的特性和发病机制。
