T 细胞因子 3（Tcf3）缺失通过诱导表观基因组修饰增加体细胞核重编程。
T-cell factor 3 (Tcf3) deletion increases somatic cell reprogramming by inducing epigenome modifications.
机构信息
Centre de Regulació Genòmica, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
出版信息
Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):11912-7. doi: 10.1073/pnas.1017402108. Epub 2011 Jul 5.
Abstract
The heterochromatin barrier must be overcome to generate induced pluripotent stem cells and cell fusion-mediated reprogrammed hybrids. Here, we show that the absence of T-cell factor 3 (Tcf3), a repressor of β-catenin target genes, strikingly and rapidly enhances the efficiency of neural precursor cell (NPC) reprogramming. Remarkably, Tcf3(-/-) ES cells showed a genome-wide increase in AcH3 and decrease in H3K9me3 and can reprogram NPCs after fusion greatly. In addition, during reprogramming of NPCs into induced pluripotent stem cells, the silencing of Tcf3 increased AcH3 and decreased the number of H3K9me3-positive heterochromatin foci early and long before reactivation of the endogenous stem cell genes. In conclusion, our data suggest that Tcf3 functions as a repressor of the reprogramming potential of somatic cells.
摘要
异染色质屏障必须被克服，才能产生诱导多能干细胞和细胞融合介导的重编程杂种。在这里，我们表明，缺乏 T 细胞因子 3（Tcf3），β-catenin 靶基因的抑制剂，显著且迅速地提高了神经前体细胞（NPC）重编程的效率。值得注意的是，Tcf3（-/-）ES 细胞显示出全基因组范围内 AcH3 的增加和 H3K9me3 的减少，并能在融合后极大地重编程 NPC。此外，在 NPC 重编程为诱导多能干细胞期间，Tcf3 的沉默在重新激活内源性干细胞基因之前很早就增加了 AcH3，并减少了 H3K9me3 阳性异染色质焦点的数量。总之，我们的数据表明 Tcf3 作为体细胞重编程潜力的抑制剂发挥作用。
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本文引用的文献
1
β-catenin enhances Oct-4 activity and reinforces pluripotency through a TCF-independent mechanism.β-catenin 通过非 TCF 依赖的机制增强 Oct-4 活性并加强多能性。
Cell Stem Cell. 2011 Feb 4;8(2):214-27. doi: 10.1016/j.stem.2010.12.010.
2
Phosphorylation of TCF proteins by homeodomain-interacting protein kinase 2.TCF 蛋白的磷酸化由同源域相互作用蛋白激酶 2 完成。
J Biol Chem. 2011 Apr 8;286(14):12093-100. doi: 10.1074/jbc.M110.185280. Epub 2011 Feb 1.
3
Regulation of TCF3 by Wnt-dependent phosphorylation during vertebrate axis specification.脊椎动物轴形成过程中 Wnt 依赖性磷酸化调控 TCF3。
Dev Cell. 2010 Oct 19;19(4):521-32. doi: 10.1016/j.devcel.2010.09.005.
4
The Wnt/β-catenin signaling pathway tips the balance between apoptosis and reprograming of cell fusion hybrids.Wnt/β-catenin 信号通路在细胞融合杂种的凋亡和重编程之间平衡中起关键作用。
Stem Cells. 2010 Nov;28(11):1940-9. doi: 10.1002/stem.515.
5
ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency.胚胎干细胞需要 PRC2 来指导分化细胞成功重编程为多能性。
Cell Stem Cell. 2010 Jun 4;6(6):547-56. doi: 10.1016/j.stem.2010.04.013.
6
Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming.BAF 复合物的染色质重塑组件有助于重编程。
Cell. 2010 Jun 11;141(6):943-55. doi: 10.1016/j.cell.2010.04.037.
7
Reprogramming towards pluripotency requires AID-dependent DNA demethylation.重编程为多能性需要 AID 依赖性的 DNA 去甲基化。
Nature. 2010 Feb 25;463(7284):1042-7. doi: 10.1038/nature08752.
8
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Stem Cells. 2009 Dec;27(12):2992-3000. doi: 10.1002/stem.240.
9
Epigenetic reprogramming and induced pluripotency.表观遗传重编程与诱导多能性。
Development. 2009 Feb;136(4):509-23. doi: 10.1242/dev.020867.
10
Periodic activation of Wnt/beta-catenin signaling enhances somatic cell reprogramming mediated by cell fusion.Wnt/β-连环蛋白信号通路的周期性激活增强了细胞融合介导的体细胞重编程。
Cell Stem Cell. 2008 Nov 6;3(5):493-507. doi: 10.1016/j.stem.2008.08.017.
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