Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan.
Genet Mol Biol. 2011 Apr;34(2):268-73. doi: 10.1590/s1415-47572011005000014. Epub 2011 Apr 1.
Genetic diversity in 403 morphologically distinct landraces of barley (Hordeum vulgare L. subsp. vulgare) originating from seven geographical zones of Asia was studied using simple sequence repeat (SSR) markers from regions of medium to high recombination in the barley genome. The seven polymorphic SSR markers representing each of the chromosomes chosen for the study revealed a high level of allelic diversity among the landraces. Genetic richness was highest in those from India, followed by Pakistan while it was lowest for Uzbekistan and Turkmenistan. Out of the 50 alleles detected, 15 were unique to a geographic region. Genetic diversity was highest for landraces from Pakistan (0.70 ± 0.06) and lowest for those from Uzbekistan (0.18 ± 0.17). Likewise, polymorphic information content (PIC) was highest for Pakistan (0.67 ± 0.06) and lowest for Uzbekistan (0.15 ± 0.17). Diversity among groups was 40% compared to 60% within groups. Principal component analysis clustered the barley landraces into three groups to predict their domestication patterns. In total 51.58% of the variation was explained by the first two principal components of the barley germplasm. Pakistan landraces were clustered separately from those of India, Iran, Nepal and Iraq, whereas those from Turkmenistan and Uzbekistan were clustered together into a separate group.
利用来自大麦基因组中中高度重组区域的简单序列重复（SSR）标记，对来自亚洲七个地理区域的 403 个形态独特的大麦农家种进行了遗传多样性研究。研究中选择的每条染色体的 7 个多态性 SSR 标记揭示了农家种之间的高水平等位基因多样性。印度的农家种遗传丰富度最高，其次是巴基斯坦，而乌兹别克斯坦和土库曼斯坦的遗传丰富度最低。在检测到的 50 个等位基因中，有 15 个是特定于一个地理区域的。巴基斯坦农家种的遗传多样性最高（0.70 ± 0.06），乌兹别克斯坦农家种的遗传多样性最低（0.18 ± 0.17）。同样，巴基斯坦的多态信息含量（PIC）最高（0.67 ± 0.06），乌兹别克斯坦的 PIC 最低（0.15 ± 0.17）。组间多样性为 40%，组内多样性为 60%。主成分分析将大麦农家种聚类为三组，以预测其驯化模式。大麦种质资源的前两个主成分共解释了 51.58%的变异。巴基斯坦农家种与印度、伊朗、尼泊尔和伊拉克的农家种分开聚类，而土库曼斯坦和乌兹别克斯坦的农家种则聚类为一个单独的组。
