Núcleo de Doenças de Transmissão Vetorial, Instituto Adolfo Lutz, São Paulo, SP.
Rev Soc Bras Med Trop. 2011 May-Jun;44(3):290-6. doi: 10.1590/s0037-86822011005000041. Epub 2011 Jul 1.
Following yellow fever virus (YFV) isolation in monkeys from the São José do Rio Preto region and two fatal human autochthonous cases from the Ribeirão Preto region, State of São Paulo, Brazil, two expeditions for entomological research and eco-epidemiological evaluation were conducted.
A total of 577 samples from humans, 108 from monkeys and 3,049 mosquitoes were analyzed by one or more methods: virus isolation, ELISA-IgM, RT-PCR, histopathology and immunohistochemical.
Of the 577 human samples, 531 were tested by ELISA-IgM, with 3 positives, and 235 were inoculated into mice and 199 in cell culture, resulting in one virus isolation. One sample was positive by histopathology and immunohistochemical. Using RT-PCR, 25 samples were processed with 4 positive reactions. A total of 108 specimens of monkeys were examined, 108 were inoculated into mice and 45 in cell culture. Four virus strains were isolated from Alouatta caraya. A total of 931 mosquitoes were captured in Sao Jose do Rio Preto and 2,118 in Ribeirão Preto and separated into batches. A single isolation of YFV was derived from a batch of 9 mosquitoes Psorophora ferox, collected in Urupês, Ribeirão Preto region. A serological survey was conducted with 128 samples from the municipalities of São Carlos, Rincão and Ribeirão Preto and 10 samples from contacts of patients from Ribeirão Preto. All samples were negative by ELISA-IgM for YFV.
The results confirm the circulation of yellow fever, even though sporadic, in the Sao Paulo State and reinforce the importance of vaccination against yellow fever in areas considered at risk.
在巴西圣保罗州圣若泽-里贝朗普雷图地区从猴子身上分离出黄热病病毒（YFV）以及两例来自里贝朗普雷图地区的本地感染人类致命病例后，进行了两次昆虫学研究和生态流行病学评估的考察。
共分析了 577 份人类样本、108 份猴子样本和 3049 份蚊子样本，采用一种或多种方法进行分析：病毒分离、ELISA-IgM、RT-PCR、组织病理学和免疫组织化学。
在 577 个人类样本中，有 531 个样本通过 ELISA-IgM 进行了检测，有 3 个呈阳性，其中 235 个接种到小鼠体内，199 个接种到细胞培养物中，结果分离出 1 株病毒。1 个样本通过组织病理学和免疫组织化学呈阳性。使用 RT-PCR 处理了 25 个样本，有 4 个呈阳性反应。共检查了 108 份猴子样本，其中 108 个接种到小鼠体内，45 个接种到细胞培养物中。从 Alouatta caraya 中分离出 4 株病毒株。在圣若泽-里贝朗普雷托共捕获了 931 只蚊子，在里贝朗普雷托共捕获了 2118 只蚊子，并将其分成几批。从里贝朗普雷托地区乌鲁佩斯采集的 9 只 Psorophora ferox 蚊子中分离出一株 YFV。对来自圣卡洛斯、林坎和里贝朗普雷托的 128 个样本以及来自里贝朗普雷托患者接触者的 10 个样本进行了血清学调查，所有样本的 YFV ELISA-IgM 均为阴性。
这些结果证实了黄热病即使是散发性的，也在圣保罗州流行，并强调了在被认为有风险的地区接种黄热病疫苗的重要性。
