Schrenzel M D, Higgins R J, Hinrichs S H, Smith M O, Torten M
Department of Pathology, School of Veterinary Medicine, University of California, Davis 95616.
Acta Neuropathol. 1990;80(5):547-53. doi: 10.1007/BF00294617.
Three cases of spontaneous olfactory neuroblastoma (ONB) in domestic cats were morphologically and immunocytochemically characterized. Diagnostic light microscopic features included Flexner and Homer-Wright rosettes, while ultrastructurally the cells had neuritic processes, intracellular intermediate filaments, and intercellular junctions. Immunocytochemically, the tumors stained positively for neuron-specific enolase, cytokeratins, and S-100 protein antigens. In each case, a key finding was the identification of numerous mature type C retroviral particles within the tumors. In one case, budding of viral particles from the plasmalemma of tumor cells suggested the source of mature particles. This cat and one other were tested, and both were serologically positive for feline leukemia virus (FeLV). The virus in the tumors was identified as FeLV by polymerase chain reaction and immunocytochemistry. No other neoplasms were found in any of the cats, nor was there similar evidence of active viral infection in other non-tumor tissues, including the brain. Although the relationship between FeLV infection and ONB is uncertain, our findings indicate that FeLV should be investigated as an etiologic agent of ONB.
对三只家猫自发性嗅神经母细胞瘤（ONB）进行了形态学和免疫细胞化学特征分析。诊断性光镜特征包括Flexner和Homer-Wright菊形团，而超微结构上细胞具有神经突、细胞内中间丝和细胞间连接。免疫细胞化学分析显示，肿瘤对神经元特异性烯醇化酶、细胞角蛋白和S-100蛋白抗原呈阳性染色。在每例中，一个关键发现是在肿瘤内鉴定出大量成熟的C型逆转录病毒颗粒。在一例中，病毒颗粒从肿瘤细胞质膜出芽提示了成熟颗粒的来源。对这只猫和另一只猫进行检测，二者血清学检测均为猫白血病病毒（FeLV）阳性。通过聚合酶链反应和免疫细胞化学鉴定肿瘤中的病毒为FeLV。在任何一只猫中均未发现其他肿瘤，在包括脑在内的其他非肿瘤组织中也没有类似的活跃病毒感染证据。尽管FeLV感染与ONB之间的关系尚不确定，但我们的研究结果表明，应将FeLV作为ONB的病因进行调查。
